Zebrafish Ephrin-B2 Antibody

Catalog # Availability Size / Price Qty
AF1088
AF1088-SP
Product Details
Citations (1)
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Zebrafish Ephrin-B2 Antibody Summary

Species Reactivity
Zebrafish
Specificity
Detects zebrafish Ephrin-B2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Ephrin-B2 is observed and less than 1% cross-reactivity with recombinant human (rh) Ephrin-A4, rmEphrin-A2, rmEphrin-A1, rhEphrin-B3, rmEphrin-B1, rhEphrin-A3, rhEphrin-A5, and rmEphrin-A4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant zebrafish Ephrin-B2
Leu25-Ala222
Accession # O73874
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Zebrafish Ephrin-B2 Fc Chimera (Catalog # 1088-B2)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-B2

Ephrin-B2 is a member of the ephrin ligand family, which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The B‑class ligands are transmembrane proteins, which can be tyrosine phosphorylated upon receptor ligation. The cytoplasmic domains are approximately 80 amino acids long and are highly conserved, especially the last 33 amino acids. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. Ephrin-B2 has been shown to bind EphA4, EphB1, EphB2, EphB3, and EphB4. Only membrane-bound or Fc‑clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation. In vivo, the ligands and receptors display reciprocal expression, indicating a general function for Eph/ephrin signaling in defining spatial boundaries in the embryo. Ephrin-B2 has been shown to play a role in a variety of developmental processes, including arterial-venous differentiation, neural crest cell migration, and axon guidance. Zebrafish Ephrin-B2 shares 66% amino acid identity with mouse Ephrin-B2. In zebrafish embryos, EphA4 and Ephrin-B2 are expressed sequentially along the anteroposterior axis of the embryo within the presomitic and somitic mesoderm. It has been shown that interference with Eph signaling leads to loss or incorrect formation of somite boundaries and disruption of myogenic differentiation.

References
  1. Eph Nomenclature Committee (1997) Cell [letter] 90(3):403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9(5):608.
  4. Durbin, L. et al. Genes & Dev. (1998) 12:3096.
  5. Adams, et al. Genes & Dev. (1999) 13:295.
Entrez Gene IDs
1948 (Human); 13642 (Mouse); 30219 (Zebrafish)
Alternate Names
EFNB2; ELF-2; ephrin B2; EphrinB2; Ephrin-B2; EPLG5; HTKL; Htk-L; LERK5; LERK-5; NLERK-1

Product Datasheets

Citation for Zebrafish Ephrin-B2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cell cycle progression is required for zebrafish somite morphogenesis but not segmentation clock function.
    Authors: Zhang L, Kendrick C, Julich D, Holley SA
    Development, 2008;135(12):2065-70.
    Species: Zebrafish
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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