ZFP200 Antibody (OTI5F4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74923

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5F4

Format

Azide and BSA Free
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Product Specifications

Immunogen

Full length human recombinant protein of human ZNF200 (NP_932353) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZFP200 Antibody (OTI5F4) - Azide and BSA Free

Western Blot: ZFP200 Antibody (OTI5F4)Azide and BSA Free [NBP2-74923]

Western Blot: ZFP200 Antibody (OTI5F4)Azide and BSA Free [NBP2-74923]

Western Blot: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF200.
Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923]

Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923]

Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923]

Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923]

Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923]

Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923]

Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923]

Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923]

Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923]

Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923]

Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for ZFP200 Antibody (OTI5F4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ZFP200

ZFP200 (ZNF200) is a gene that codes for a protein with three isoforms, with lengths of 395, 394 and 394 amino acids and weights of approximately 46, 45, and 45 kDa respectively. ZFP200 is highly expressed in the testis and could have a role in spermatogenesis. Current studies are being done on diseases and disorder related to this gene including familial Mediterranean fever, anemia, and prostatitis. ZFP200 has also been shown to have interactions with PPARGC1B and UBC in pathways such as the generic transcription and gene expression pathways.

Alternate Names

MGC45293, zinc finger protein 200, ZNFMF

Gene Symbol

ZNF200

Additional ZFP200 Products

Product Documents for ZFP200 Antibody (OTI5F4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ZFP200 Antibody (OTI5F4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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