ZIC3 Antibody - BSA Free

Western Blot: ZIC3 Antibody - BSA Free [NBP1-33207] -

Zic3-ApoM-Nnt axis mediates cell homeostasis between mitophagy and apoptosis.A, C, D C17.2 cells were transfected with Ncontrol (NC) or siRNA targeting Zic3 (Zic3-si). B, E C17.2 cells were transfected with empty pcDNA vector (Vector) or WT Zic3 cDNA (Zic3-oe). A, B PINK1, PRKN and LC3-II/LC3-I protein levels in mitochondria were measured by western blotting (n = 3 from independent experiments). C C17.2 cells were double IF-stained with LC3-labelled autophagosomes (green) and TOMM20-labelled mitochondria (red). Nuclei were counterstained with DAPI. Scale bar = 10 um. D, E Nnt, Bax/Bcl-2, cleaved Caspase 9, and cleaved Caspase 3 protein levels were analysed by western blotting (n = 3 independent experiments). F, G PINK1, PRKN and LC3-II/LC3-I protein levels in mitochondria were analysed by western blotting under different transfection conditions in C17.2 cells (n = 3 from independent experiments). H, I Nnt, cleaved Caspase 9, cleaved Caspase 3, and Bax/Bcl-2 protein levels were analysed by western blotting under different transfection conditions in C17.2 cells (n = 3 from independent experiments). GAPDH or VDAC1 was used as a loading control. Data are expressed as the mean +/- SD. Statistical comparisons were performed using Student’s t tests in (A, B, D and E); one-way ANOVA in (F and G). *P < 0.05; **P < 0.01; “ns" represents no significance vs. the indicated group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39827160), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ZIC3 Antibody - BSA Free [NBP1-33207] -

Zic3-ApoM-Nnt axis mediates cell homeostasis between mitophagy and apoptosis.A, C, D C17.2 cells were transfected with Ncontrol (NC) or siRNA targeting Zic3 (Zic3-si). B, E C17.2 cells were transfected with empty pcDNA vector (Vector) or WT Zic3 cDNA (Zic3-oe). A, B PINK1, PRKN and LC3-II/LC3-I protein levels in mitochondria were measured by western blotting (n = 3 from independent experiments). C C17.2 cells were double IF-stained with LC3-labelled autophagosomes (green) and TOMM20-labelled mitochondria (red). Nuclei were counterstained with DAPI. Scale bar = 10 um. D, E Nnt, Bax/Bcl-2, cleaved Caspase 9, and cleaved Caspase 3 protein levels were analysed by western blotting (n = 3 independent experiments). F, G PINK1, PRKN and LC3-II/LC3-I protein levels in mitochondria were analysed by western blotting under different transfection conditions in C17.2 cells (n = 3 from independent experiments). H, I Nnt, cleaved Caspase 9, cleaved Caspase 3, and Bax/Bcl-2 protein levels were analysed by western blotting under different transfection conditions in C17.2 cells (n = 3 from independent experiments). GAPDH or VDAC1 was used as a loading control. Data are expressed as the mean +/- SD. Statistical comparisons were performed using Student’s t tests in (A, B, D and E); one-way ANOVA in (F and G). *P < 0.05; **P < 0.01; “ns" represents no significance vs. the indicated group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39827160), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ZIC3 Antibody - BSA Free [NBP1-33207] -

Zic3-ApoM-Nnt axis mediates cell homeostasis between mitophagy and apoptosis.A, C, D C17.2 cells were transfected with Ncontrol (NC) or siRNA targeting Zic3 (Zic3-si). B, E C17.2 cells were transfected with empty pcDNA vector (Vector) or WT Zic3 cDNA (Zic3-oe). A, B PINK1, PRKN and LC3-II/LC3-I protein levels in mitochondria were measured by western blotting (n = 3 from independent experiments). C C17.2 cells were double IF-stained with LC3-labelled autophagosomes (green) and TOMM20-labelled mitochondria (red). Nuclei were counterstained with DAPI. Scale bar = 10 um. D, E Nnt, Bax/Bcl-2, cleaved Caspase 9, and cleaved Caspase 3 protein levels were analysed by western blotting (n = 3 independent experiments). F, G PINK1, PRKN and LC3-II/LC3-I protein levels in mitochondria were analysed by western blotting under different transfection conditions in C17.2 cells (n = 3 from independent experiments). H, I Nnt, cleaved Caspase 9, cleaved Caspase 3, and Bax/Bcl-2 protein levels were analysed by western blotting under different transfection conditions in C17.2 cells (n = 3 from independent experiments). GAPDH or VDAC1 was used as a loading control. Data are expressed as the mean +/- SD. Statistical comparisons were performed using Student’s t tests in (A, B, D and E); one-way ANOVA in (F and G). *P < 0.05; **P < 0.01; “ns" represents no significance vs. the indicated group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39827160), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ZIC3 Antibody - BSA Free [NBP1-33207] -

Zic3-ApoM-Nnt axis mediates cell homeostasis between mitophagy and apoptosis.A, C, D C17.2 cells were transfected with Ncontrol (NC) or siRNA targeting Zic3 (Zic3-si). B, E C17.2 cells were transfected with empty pcDNA vector (Vector) or WT Zic3 cDNA (Zic3-oe). A, B PINK1, PRKN and LC3-II/LC3-I protein levels in mitochondria were measured by western blotting (n = 3 from independent experiments). C C17.2 cells were double IF-stained with LC3-labelled autophagosomes (green) and TOMM20-labelled mitochondria (red). Nuclei were counterstained with DAPI. Scale bar = 10 um. D, E Nnt, Bax/Bcl-2, cleaved Caspase 9, and cleaved Caspase 3 protein levels were analysed by western blotting (n = 3 independent experiments). F, G PINK1, PRKN and LC3-II/LC3-I protein levels in mitochondria were analysed by western blotting under different transfection conditions in C17.2 cells (n = 3 from independent experiments). H, I Nnt, cleaved Caspase 9, cleaved Caspase 3, and Bax/Bcl-2 protein levels were analysed by western blotting under different transfection conditions in C17.2 cells (n = 3 from independent experiments). GAPDH or VDAC1 was used as a loading control. Data are expressed as the mean +/- SD. Statistical comparisons were performed using Student’s t tests in (A, B, D and E); one-way ANOVA in (F and G). *P < 0.05; **P < 0.01; “ns" represents no significance vs. the indicated group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39827160), licensed under a CC-BY license. Not internally tested by Novus Biologicals.