ZKSCAN1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81346

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ZKSCAN1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NLARRNLSRDNRQENYGSAFPQGGENRNENEESTSKAETSEDSASRGETTGRSQKEFGEKRDQEGKTGERQQKNPEEKTRKEKRDSGPAIGKDKKTITGERGPREKGKGLGRSFSLSSNFTTPEEVPTGTKSHRCDECGKCFTR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZKSCAN1 Antibody - BSA Free

Western Blot: ZKSCAN1 Antibody [NBP1-81346]

Western Blot: ZKSCAN1 Antibody [NBP1-81346]

Western Blot: ZKSCAN1 Antibody [NBP1-81346] - Analysis in human cell line SH-SY5Y.
Immunocytochemistry/ Immunofluorescence: ZKSCAN1 Antibody [NBP1-81346]

Immunocytochemistry/ Immunofluorescence: ZKSCAN1 Antibody [NBP1-81346]

Immunocytochemistry/Immunofluorescence: ZKSCAN1 Antibody [NBP1-81346] - Staining of human cell line A-431 shows localization to nucleus & nuclear bodies.
Immunohistochemistry-Paraffin: ZKSCAN1 Antibody [NBP1-81346]

Immunohistochemistry-Paraffin: ZKSCAN1 Antibody [NBP1-81346]

Immunohistochemistry-Paraffin: ZKSCAN1 Antibody [NBP1-81346] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
ZKSCAN1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: ZKSCAN1 Antibody - BSA Free [NBP1-81346]

Chromatin Immunoprecipitation-exo-Seq: ZKSCAN1 Antibody - BSA Free [NBP1-81346]

ChIP-Exo-Seq composite graph for Anti-ZKSCAN1 (NBP1-81346) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for ZKSCAN1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZKSCAN1

ZKSCAN1 is a zinc-finger protein that contains six C2H2-type zinc fingers, a SCAN box domain, and a KRAB (kruppel-associated box) domain. Due to its structure, the function of ZKSCAN1is predicted to be involved in transcription. The KRAB domain is found in many C2H2-type zinc fingers and may be involved in mediating protein-protein reactions. The SCAN domain (named after SRE-ZBP, CTfin51, AW-1, and Number 18 cDNA) may also be involved in protein interactions due to its ability to mediate homo- and hetero-oligomerization. Alternative names for ZKSCAN1 include zinc finger protein with KRAB and SCAN domains 1, KOX18, ZNF139, ZNF36, and PHZ-37.

Alternate Names

KOX189130423L19Rik, PHZ-37, Zinc finger protein 139, Zinc finger protein 36, zinc finger protein 36 (KOX 18), Zinc finger protein KOX18, zinc finger protein with KRAB and SCAN domains 1, zinc finger with KRAB and SCAN domains 1, ZNF139, ZNF36MGC138429

Gene Symbol

ZKSCAN1

Additional ZKSCAN1 Products

Product Documents for ZKSCAN1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ZKSCAN1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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