ZNF276 Antibody - BSA Free

Immunohistochemistry: ZNF276 Antibody - BSA Free [NBP1-82681] -

ZNF276 activates the Wnt/ beta -catenin signaling by regulation of CYP1B1.A, C Western blotting (A) and RT-qPCR (C) analysis revealed that silencing of CYP1B1 inhibited the expression of c-Myc and Cyclin D1 enhanced by ZNF276 overexpression in MDA-MB-231 cells. B, D Effects of ZNF276 knockdown and CYP1B1 overexpression on c-Myc and Cyclin D1 expression were analyzed in UACC-812 cells by western blotting (B) and RT-qPCR (D). E, F ZNF276 lacking C2H2 domain inhibited the TOP Flash activity enhanced by the intact ZNF276 with wnt3a activation in MDA-MB-231 (E) and UACC-812 (F) cells. G Endogenous beta -catenin expression and distribution was observed by immunofluorescence in MDA-MB-231 cells transfected with intact ZNF276 or ZNF276 lacking C2H2 domain plasmid. Nuc nuclear. Bar = 20 um. *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36085146), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: ZNF276 Antibody - BSA Free [NBP1-82681] -

ZNF276 activates Wnt/ beta -catenin signaling through recruitment of MAGEB2 to regulate CYP1B1 transcription.A GO analysis of the potential ZNF276-interacted proteins. B KEGG analysis of the potential ZNF276-interacted proteins. C, D Both forward (C) and reverse (D) co-IP analysis affirmed the interaction between ZNF276 and MAGEB2. E ChIP-PCR analysis of ZNF276 binding ability at the CYP1B1 promoter affected by MAGEB2 overexpression. F, G The luciferase activity of CYP1B1 promoter decreased by ZNF276 silencing was further enhanced by the expression of MAGEB2 in 293T and MCF-7. H, I RT-qPCR (H) and western blot (I) analysis confirmed the effect of MAGEB2 overexpression in CYP1B1 mRNA and protein expression in MCF-7 cells. J, K The reduced transcriptional activity of CYP1B1 promoter by ZNF276 was partially reversed by MAGEB2. L, M Overexpression of MAGEB2 reversed the decreased cell proliferation, migration and invasion abilities affected by ZNF276 silencing in CCK-8 assay (L), transwell migration (M, up) and transwell invasion (M, down). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36085146), licensed under a CC-BY license. Not internally tested by Novus Biologicals.