ZNF295 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-74655
![Western Blot: ZNF295 Antibody [NB100-74655]](https://resources.rndsystems.com/images/products/ZNF295-Antibody-Western-Blot-NB100-74655-img0001.jpg "Western Blot: ZNF295 Antibody [NB100-74655]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 875 and 925 of human zinc finger protein 295 using the numbering given in entry NP_065778.3 (GeneID 49854).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ZNF295 Antibody - BSA Free
Western Blot: ZNF295 Antibody [NB100-74655]
Western Blot: ZNF295 Antibody [NB100-74655] - Detection of Human ZNF295 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZNF295 antibody NB100-74655 used for IP at 3 mcg/mg lysate. ZNF295 was also immunoprecipitated by rabbit anti-ZNF295 antibody NB100-74656, which recognizes a downstream epitope.![Immunocytochemistry/ Immunofluorescence: ZNF295 Antibody [NB100-74655]](https://resources.rndsystems.com/images/products/ZNF295-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74655-img0003.jpg "Immunocytochemistry/ Immunofluorescence: ZNF295 Antibody [NB100-74655]")
Immunocytochemistry/ Immunofluorescence: ZNF295 Antibody [NB100-74655]
Immunocytochemistry/Immunofluorescence: ZNF295 Antibody [NB100-74655] - Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100![Immunohistochemistry: ZNF295 Antibody [NB100-74655]](https://resources.rndsystems.com/images/products/ZNF295-Antibody-Immunohistochemistry-NB100-74655-img0002.jpg "Immunohistochemistry: ZNF295 Antibody [NB100-74655]")
Immunohistochemistry: ZNF295 Antibody [NB100-74655]
Immunohistochemistry: ZNF295 Antibody [NB100-74655] - Sample: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZNF295 used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5ug/ml).Applications for ZNF295 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:250-1:1000
Immunohistochemistry-Paraffin
1:500-1:2000
Immunoprecipitation
2-5ug/mg Lysate
Application Notes
This antibody is not recommended for Western Blot analysis.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ZNF295
Alternate Names
MGC15716, ZFP414, zinc finger protein 414
Entrez Gene IDs
49854 (Human)
Gene Symbol
ZNF295
UniProt
Additional ZNF295 Products
Product Documents for ZNF295 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ZNF295 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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