ZNF674 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-93452

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ZNF674 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IKHQRTHTGEKPFVCDKCPKAFKSSYHLIRHEKTHIRQAFYKGIKCTTSSLIYQRIHTSEKPQCSEHGKASDEKPSPT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF674 Antibody - BSA Free

Immunohistochemistry-Paraffin: ZNF674 Antibody [NBP1-93452]

Immunohistochemistry-Paraffin: ZNF674 Antibody [NBP1-93452]

Immunohistochemistry-Paraffin: ZNF674 Antibody [NBP1-93452] - Staining of human testis shows distinct positivity in spermatozoa.
ZNF674 Antibody - BSA Free Western Blot: ZNF674 Antibody - BSA Free [NBP1-93452]

Western Blot: ZNF674 Antibody - BSA Free [NBP1-93452]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
ZNF674 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: ZNF674 Antibody - BSA Free [NBP1-93452]

Chromatin Immunoprecipitation-exo-Seq: ZNF674 Antibody - BSA Free [NBP1-93452]

ChIP-Exo-Seq composite graph for Anti-ZNF674 (NBP1-93452) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for ZNF674 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF674

Alternate Names

MRX92, zinc finger protein 674

Gene Symbol

ZNF674

Additional ZNF674 Products

Product Documents for ZNF674 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ZNF674 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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