Western Blot

Western Blot Conditions

Buffer Group 1

Blotting Buffer Blocking Solution Antibody Solution
25 mM Tris, pH 7.4, 0.15 M NaCl, 0.1% Tween 20 5% nonfat dry milk in Blotting Buffer, Adjust pH to 7.4 5% nonfat dry milk in Blotting Buffer, Adjust pH to 7.4

 

Buffer Group 2

 

Dot Blot Protocol

A Dot Blot is a simple and quick assay that may be employed to determine if your antibodies and detection system are effective. Dot Blot may also be used to determine appropriate starting concentration of primary antibody for Western blot.

Western Blot Protocol for Cell Lysates

This protocol is intended to provide a set of initial conditions for analysis of cell lysates samples by Western blot. Further optimization may be required for individual samples or analytes. Follow manufacturer's protocols for specific reagents when applicable.

Western Blot Protocols- Quality Control Protocol

R&D Systems Quality Control laboratories use these Western blotting and immunostaining protocols to show that our polyclonal and monoclonal antibodies are specific for the proteins they were raised against and to determine the sensitivity of the antibody for its antigen.