Figure 1 – Effect of Varying Primary Antibody Concentration:
Western blot optimization using R&D Systems rabbit anti-human/mouse/rat RSK1 affinity purified polyclonal antibody (Catalog # AF992). Human HeLa cells were solubilized in SDS gel buffer, and lysate from 1 x 105 cells per lane was resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane (Millipore) and immunoblotted under the following conditions. A 30-second exposure to film is shown.