|Figure 1. eNOS detection in human paraffin-embedded placenta tissue section using R&D Systems’ goat anti-human eNOS antibody (Catalog # AF950). Chromogenic detection was done using R&D Systems’ Cell and Tissue anti-goat AEC Staining Kit (Catalog # CTS009). Tissue was counterstained with Hematoxylin.||Figure 2. Sonic Hedgehog (Shh) labeling in rat cerebellum was done using R&D Systems’ goat anti-mouse Shh antibodies (Catalog # AF445) and HRP-DAB (brown color) Cell and Tissue Staining Kit (Catalog # CTS008). R&D Systems’ Antigen Retrieval Reagent-Basic (Catalog # CTS013) was used to unmask Shh antigen. Tissue was countertstained using Hematoxylin (blue color).|
R&D Systems’ Cell and Tissue Staining Kits detect goat, mouse, and rabbit primary antibodies using HRP-DAB, HRP-AEC, or AP-BCIP/NBT enzymatic systems. The high detection sensitivity of these staining kits is achieved by using high quality secondary biotinylated antibodies and high sensitivity streptavidin (HSS) conjugated to horseradish peroxidase (HRP) or alkaline phosphatase (AP). HSS is a chemical analog of streptavidin that has little net positive charge at neutral or slightly alkaline pH and will only interact with biotin attached to secondary antibodies. HSS-HRP shows little or no non-specific binding to phospholipids, nucleic acids and carbohydrate binding proteins.
R&D Systems’ Cell and Tissue Staining Kits are optimized for use on cryostat and paraffin-embedded tissue sections, cytospin preparations (i.e., lymphocytes, monocytes, transfected cells) and free-floating tissue sections. These kits may be used with primary antibodies manufactured by either R&D Systems or other vendors. The kits are made in a ready-to-use format that eliminates the need for extra steps in the procedure, minimizes hands-on time, maximizes convenience and reduces the risk of erroneous calculations.