Get the most out of your primary antibodies!
Designed to optimize staining on cryostat, paraffin-embedded, and free-floating tissue sections, these kits provide all the reagents required to maximize your IHC experiments.
Key Benefits
- Ready to use
- High Sensitivity Streptavidin (HSS) increases detection sensitivity
- Suitable for tissue sections & cytospin preparations
- Pre-diluted reagents minimize hands-on time
- Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining
Kit Components for IHC Optimization
Blocking reagents
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To minimize non-specific Peroxidase, Avidin, and Biotin signals
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Secondary antibodies
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Biotinylated and pre-diluted to minimize hands-on time
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HSS-conjugated HRP
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Chemically optimized Streptavidin displays little or no non-specific binding to phospholipids, nucleic acids, and carbohydrate binding proteins
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Chromogen
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Choose DAB (brown) or AEC (red)
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Data Examples
GALNT10 in Mouse Testis. Optimized using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (Catalog # CTS019).
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VSIG4 in Mouse Liver. Optimized using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (Catalog # CTS008).
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IA-2/PTPRN in Human Pancreas. Optimized using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (Catalog # CTS002).
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NT-3 in Rat Brain. Optimized using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (Catalog # CTS009).
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Figure Legends
Polypeptide GalNac Transferase 10/GALNT10 in Mouse Testis. Polypeptide GalNac Transferase 10/GALNT10 was detected in perfusion fixed frozen sections of mouse testis using Sheep Anti-Human/Mouse Polypeptide GalNac Transferase 10/GALNT10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7575) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Golgi organelles in spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
VSIG4 in Mouse Liver. V-set and immunoglobulin domain containing 4 (VSIG4) was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse VSIG4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4674) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Kuppfer cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
IA-2/PTPRN in Human Pancreas. Islet Cell Antigen 2 (IA-2)/Protein Tyrosine Phosphatase Receptor-type N (PTPRN) was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human IA-2/PTPRN Monoclonal Antibody (Catalog # MAB7906) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
NT-3 in Rat Brain. NT-3 was detected in perfusion fixed frozen sections of rat brain (Purkinje cells in the cerebellum) using 10 µg/mL Human NT-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-267-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Intended for the localization of antigens in a broad range of histological and cytological specimens, these kits may b used with primary IgG antibodies manufacturd by R&D System or by other vendors. Briefly, detection is based on the formation of the Avidin-Biotin Complex (ABC). Visualization is based on enzymatic conversion of chromogenic substrates 3,3’ Diaminobenzidine (DAB) or 3-amino-9-ethylcarbazole (AEC) into a colored precipitate (brown or red, respectively), by horseradish peroxidase (HRP) at the sites of antigen localization, which can then be viewed using bright-field microscopy.
The high detection sensitivity of R&D Systems Cell and Tissue Staining Kits is achieved by using premium quality biotinylated secondary antibodies and High Sensitivity Streptavidin-conjugated HRP (HSS-HRP). HSS is a chemical analog of Streptavidin that has little net positive charge at neutral or slightly alkaline pH and will interact only with Biotin attached to secondary antibodies. HSS-HRP shows little or no non-specific binding to phospholipids, nucleic acids, and carbohydrate binding proteins.