PlGF ELISA for Preeclampsia Research

Figure 1. Typical standard curve generated using R&D Systems Human PlGF Quantikine ELISA (Catalog # DPG00). These data are for demonstration purposes only. A standard curve should be generated for each set of samples assayed.
Figure 2. Human serum, plasma, and urine samples were assayed for PlGF using R&D Systems Human PlGF Quantikine ELISA (Catalog # DPG00). Values represent the mean obtained from 18 or more samples each.

Preeclampsia is a serious medical condition found in approximately 5% of pregnant women. Although it is not usually fatal, it is characterized by hypertension, proteinuria, and edema. Complications can include seizures, stroke, and kidney and liver failure. Researchers have hypothesized that preeclampsia results from an imbalance of angiogenic factors and recent studies show that serum, plasma, and urine PlGF levels decrease significantly in women with preeclampsia and/or those who subsequently develop the disorder.

R&D Systems Quantikine® Human PlGF ELISA is now fully-validated for urine as well as serum, plasma, and cell culture supernate samples. This assay has a sensitivity of less than 7 pg/mL and a dynamic range of 15.6 to 1,000 pg/mL.

Selected citations using R&D Systems Human PlGF Quantikine ELISA in Preeclampsia Research

  • Levine, R.J. et al. (2005) Urinary placental growth factor and risk for preeclampsia. JAMA 293:77.
    Sample Type: Human urine
  • Lambert-Messerlian, G.M. & J.A. Canick (2004) Placenta growth factor levels in second-trimester maternal serum in Down syndrome pregnancy and in the prediction of preeclampsia. Prenat. Diagn. 24:876.
    Sample Type: Human serum
  • Levine, R.J. et al. (2004) Circulating angiogenic factors and the risk of preeclampsia. N. Engl. J. Med. 350:672.
    Sample Type: Human serum
  • Nagamatsu, T. et al. (2004) Cytotrophoblasts up-regulate soluble fms-like tyrosine kinase-1 expression under reduced oxygen: an implication for the placental vascular development and the pathophysiology of preeclampsia. Endocrinology 145:4835.
    Sample Type: Human cytotrophoblast culture supernatant