Two subsets of lymphoid tissue-resident classical dendritic cell (cDC) subsets have been characterized in mice, CD8 alpha+ cDCs and CD11b+ cDCs. While both of these subsets express CD11c and MHC class II, they can be distinguished from each other based on a number of other markers. CD8 alpha+ cDCs express CD8 alpha, CLEC9a, IGSF4A/SynCAM1/Necl2, and XCR1 and lack expression of CD11b/Integrin alpha M, DCIR-2, DC-SIGN/CD209, F4/80, and SIRP alpha/CD172. In contrast, CD11b+ cDCs express CD11b/Integrin alpha M, DCIR2, DC-SIGN/CD209, F4/80, and SIRP alpha/CD172a and lack expression of CD8 alpha, CLEC9a, IGSF4A/SynCAM1/Necl2, and XCR1. Functionally, these two subsets are also distinct. CD8 alpha+ cDCs are known to cross-present antigens to CD8+ T cells and prime Th1 and cytotoxic T cell responses, while CD11b+ cDCs preferentially activate CD4+ T cells and promote Th2- or Th17-driven immune responses.
Human and Mouse Dendritic Cell Subsets Poster