Embryonic stem cells (ESCs) have the exceptional ability to both self-renew and differentiate into nearly every cell of the human body. Induced pluripotent stem cells (iPSCs) are somatic cells that have been reprogrammed back into an ESC-like phenotype. Both ESCs and iPSCs have numerous roles to play in drug discovery studies, understanding mechanisms of disease, cell therapies, and developmental biology.
Expression of Pluripotency Markers in Human Embryonic Stem Cells. Pluripotency marker expression was detected in immersion-fixed BG01V human embryonic stem cells using antibodies supplied in the Human Pluripotent Stem Cell Markers Antibody Panel (R&D Systems, Catalog # SC008). Pluripotency marker expression was analyzed by dual immunofluorescence with the indicated primary antibodies supplied in the panel. The cells were stained using NorthernLights™ (NL) 493- and NL557-conjugated Secondary Antibodies (green and red, respectively). Where indicated, the nuclei were counterstained with DAPI (blue).
Verification of Pluripotency in Human Induced Pluripotent Stem Cells. iPS2 human induced pluripotent stem cells were grown on irradiated mouse embryonic fibroblasts (R&D Systems, Catalog # PSC001) and stained using antibodies included in the GloLIVE™ Human Pluripotent Stem Cell Live Cell Imaging Kit (R&D Systems, Catalog # SC023B). A. iPS2 cells stained with the NL493-conjugated SSEA-4 (green) and the NL557-conjugated SSEA-1 (red) antibodies. B. iPS2 cells stained with the NL493-conjugated SSEA-4 (green) and the N557-conjugated TRA-1-60(R) (red) antibodies. The cells were counterstained with Hoechst 33342 (blue). The colonies are positive for the stem cell markers SSEA-4 and TRA-1-60(R) and are negative for SSEA-1, suggesting that these colonies primarily contain undifferentiated human stem cells.