Mesenchymal stem cells (MSCs) are progenitor cells found in the bone marrow and other tissues. These multipotent, self-renewing cells give rise to osteoblasts, chondrocytes, myocytes, and adipocytes. Levels of these progenitor cells play a role in regenerative medicine, immune system modulation, and other cell therapies.
Detection of Cell Surface Markers on Human Bone Marrow-derived Mesenchymal Stem Cells by Flow Cytometry. Human bone marrow-derived mesenchymal stem cells were stained using reagents supplied in the Human Mesenchymal Stem Cell Verification Multi-Color Flow Cytometry Kit (R&D Systems, Catalog # FMC020). The data shows positive expression of the mesenchymal stem cell-associated surface antigens, 5'Nucleotidase/CD73, CD105/Endoglin, and CD90/Thy1, as well as negative expression of all markers included in the negative marker cocktail including CD11b/Integrin alpha M, CD34, CD45, CD79A, and HLA-DR. Quadrants were set based on staining with the appropriate negative controls.