How to Reconstitute Lyophilized Proteins

Key protocol steps:

  1. Allow the vial and reconstitution buffer to equilibrate to room temperature.
  2. Briefly centrifuge or tap down the vial to ensure that all lyophilisate is collected at the bottom of the vial.
  3. Add the amount of buffer required to achieve the concentration recommended on the product insert or Certificate of Analysis.
  4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and protein denaturation.
  5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4 °C overnight.


Utilize our Calculators

View our Reconstitution Buffers

If product does not go into solution or if you have any questions about your reconstitution, please contact R&D Systems Technical Service at