Induction and Analysis of Epithelial to Mesenchymal Transition
Yixin Tang1, Greg Herr2, Wade Johnson2, Ernesto Resnik1, and Joy Aho2
1Antibody Development Department 2Stem Cells and Developmental Biology Dept. R&D Systems
Sep 19, 2013
Epithelial to mesenchymal transition (EMT) is essential for proper morphogenesis during development. Misregulation of this process has been implicated as a key event in fibrosis and the progression of carcinomas to a metastatic state. Understanding the processes that underlie EMT is imperative for the early diagnosis and clinical control of these disease states. Reliable induction of EMT in vitro is a useful tool for drug discovery as well as to identify common gene expression signatures for diagnostic purposes. Here we demonstrate a straightforward method for the induction of EMT in a variety of cell types. Methods for the analysis of cells pre- and post-EMT induction by immunocytochemistry are also included. Additionally, we demonstrate the effectiveness of this method through antibody-based array analysis and migration/invasion assays.