DermaTACs In Situ Apoptosis Detection Kit

R&D Systems | Catalog # 4829-30-K

R&D Systems
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Key Product Details

Features

Provides researchers with an effective method for measuring apoptosis in skin samples using DNA end-labeling using terminal deoxynucleotidyl transferase (TdT), modified nucleotides (BrdU).

Key Benefits

  • Performance tested on dermal tissues.
  • Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagents.
  • Helps resolve unique problems encountered when detecting apoptosis in dermal tissue.

Species

Multi-Species

Product Summary for DermaTACs In Situ Apoptosis Detection Kit

Why Use the DermaTACS In Situ Apoptosis Detection Kit?
The DermaTACS Kit was developed to provide researchers with an effective method for measuring apoptosis in skin samples. The kit was based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT), modified nucleotides (BrdU). Detection of incorporated molecules is achieved using a biotinylated BrdU antibody and chromogenic substrate with a Streptavidin horseradish peroxidase detection system.

The extracellular scaffolding in skin tissue can make it problematic to permeabilize and high background is a common problem with skin samples. The DermaTACS Kit includes two permeabilization reagents as options for permeabilization along with a detailed protocol with hints and tips for optimal labeling of skin samples based on empirical testing. Low background is achieved using a combination of optimized reagents and visualization methods. Also included is TACS-Nuclease to generate a positive control from your own sample. In conjunction with the excellent contrast between the TACS Blue Label and the Red Counterstain C, the positive control ensures easy data interpretation.

Kit Contents for DermaTACs In Situ Apoptosis Detection Kit

  • Streptavidin-HRP
  • TACS-Blue Label
  • TACS-Nuclease
  • TACS-Nuclease Buffer
  • Red Counterstain C
  • TACS 2 TdT Labeling Buffer
  • TACS 2 TdT Stop Buffer
  • TdT Enzyme
  • B-dNTP Mix
  • anti-BrdU antibody
  • Strep-Diluent
  • Cytonin

Formulation, Preparation, and Storage

Shipping

The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Product Documents for DermaTACs In Situ Apoptosis Detection Kit

Certificate of Analysis

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Citations for DermaTACs In Situ Apoptosis Detection Kit

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  • DermaTACs In Situ Apoptosis Detection Kit
    Name: Anonymous
    Verified Customer | Posted 04/13/2018
    This kit was ordered from Trevigen (order number 64163) and used a few years ago. It was the positive control using Proteinase K and TACS-Nuclease in mouse skin.
    DermaTACs In Situ Apoptosis Detection Kit 4829-30-K

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