8-oxo-dG Antibody (15A3)

R&D Systems | Catalog # 4354-MC-050

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse, Rat, Drosophila, Insect - Drosophila, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, ELISA, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP), Bioassay, Co-Immunoprecipitation, Comet Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 15A3
View all 8-oxo-dG Primary Antibodies »

Product Specifications

Immunogen

8-oxo-dG-conjugated-KLH

Specificity

This mouse monoclonal antibody specifically binds to 8-hydroxy-2'- deoxyguanosine within DNA in H

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for 8-oxo-dG Antibody (15A3)

Detection of Rat 8-oxo-dG 8-oxo-dG Antibody (15A3) by Immunohistochemistry

Detection of Rat 8-oxo-dG 8-oxo-dG Antibody (15A3) by Immunohistochemistry

Expression of apoptotic factors in denervated muscle 2 weeks after denervation.(A) Expression of Bcl-2, Bad, and Bax in denervated muscle subjected to different treatment (B) Quantitative analysis of apoptotic markers in different treatment groups (C) The expression of 8-oxo-dG in normal, control, ES, and AFS group. (D) The representative of western blot analysis of 8-oxo-dG related to different treatment (n = 2) (F) Quantitative analysis of caspase-3 in different treatment groups Bar length = 100 μm. R = right, L = left, N = 6 for each group, * p<0.05 and **p<0.01indicated a statistical difference compared to the control group; # p<0.05 indicated a statistical difference as compared to the ES group. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25945496), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat 8-oxo-dG 8-oxo-dG Antibody (15A3) by Western Blot

Detection of Rat 8-oxo-dG 8-oxo-dG Antibody (15A3) by Western Blot

Expression of apoptotic factors in denervated muscle 2 weeks after denervation.(A) Expression of Bcl-2, Bad, and Bax in denervated muscle subjected to different treatment (B) Quantitative analysis of apoptotic markers in different treatment groups (C) The expression of 8-oxo-dG in normal, control, ES, and AFS group. (D) The representative of western blot analysis of 8-oxo-dG related to different treatment (n = 2) (F) Quantitative analysis of caspase-3 in different treatment groups Bar length = 100 μm. R = right, L = left, N = 6 for each group, * p<0.05 and **p<0.01indicated a statistical difference compared to the control group; # p<0.05 indicated a statistical difference as compared to the ES group. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25945496), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat 8-oxo-dG by Western Blot

Detection of Rat 8-oxo-dG by Western Blot

Expression of apoptotic factors in denervated muscle 2 weeks after denervation.(A) Expression of Bcl-2, Bad, and Bax in denervated muscle subjected to different treatment (B) Quantitative analysis of apoptotic markers in different treatment groups (C) The expression of 8-oxo-dG in normal, control, ES, and AFS group. (D) The representative of western blot analysis of 8-oxo-dG related to different treatment (n = 2) (F) Quantitative analysis of caspase-3 in different treatment groups Bar length = 100 μm. R = right, L = left, N = 6 for each group, * p<0.05 and **p<0.01indicated a statistical difference compared to the control group; # p<0.05 indicated a statistical difference as compared to the ES group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25945496), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat 8-oxo-dG by Immunocytochemistry/ Immunofluorescence

Detection of Rat 8-oxo-dG by Immunocytochemistry/ Immunofluorescence

Expression of apoptotic factors in denervated muscle 2 weeks after denervation.(A) Expression of Bcl-2, Bad, and Bax in denervated muscle subjected to different treatment (B) Quantitative analysis of apoptotic markers in different treatment groups (C) The expression of 8-oxo-dG in normal, control, ES, and AFS group. (D) The representative of western blot analysis of 8-oxo-dG related to different treatment (n = 2) (F) Quantitative analysis of caspase-3 in different treatment groups Bar length = 100 μm. R = right, L = left, N = 6 for each group, * p<0.05 and **p<0.01indicated a statistical difference compared to the control group; # p<0.05 indicated a statistical difference as compared to the ES group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25945496), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for 8-oxo-dG Antibody (15A3)

Application
Recommended Usage

ELISA

Empirical determination will be required for optimal results.

Immunocytochemistry

1:250 dilution
Sample:

H2O2 treated MCF 10A human breast epithelial cell line

Immunohistochemistry

1:250 dilution
Sample: Paraffin embedded rat thymus tissue

Reviewed Applications

Read 4 reviews rated 4.8 using 4354-MC-050 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

This antibody is provided as purified immunoglobulin from mouse ascites at 0.5 mg/ml in 1X PBS containing 0.1% sodium azide, 50% glycerol.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: 8-oxo-dG

The production of 8-Hydroxyguanine (8-oxo-dG) is almost exclusively elicited by oxidative stress. Polymerases preferentially insert adenine opposite 8-oxo-dG. Therefore, oxidatively damaged adducts, without repair, are susceptible to G to T transitions.

Long Name

8-Hydroxyguanine

Alternate Names

8-OHG, 8oxodG

Additional 8-oxo-dG Products

Product Documents for 8-oxo-dG Antibody (15A3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for 8-oxo-dG Antibody (15A3)

For research use only

Related Research Areas

Base Excision Repair

Citations for 8-oxo-dG Antibody (15A3)

Customer Reviews for 8-oxo-dG Antibody (15A3) (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
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1 Stars
0%

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Customer Images

Showing  1 - 4 的 4 reviews Showing All
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  • 8-oxo-dG Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Stomach tissue
    Species: Mouse
    Verified Customer | Posted 01/03/2022
    8-oxo-dG Antibody (15A3) 4354-MC-050
  • 8-oxo-dG Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 10/28/2021
    Blue- DAPI; Green:8-oxo-dG
    8-oxo-dG Antibody (15A3) 4354-MC-050
  • 8-oxo-dG Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: prostate carcinoma
    Species: Human
    Verified Customer | Posted 08/11/2021
    8-oxo-dG Antibody (15A3) 4354-MC-050
  • 8-oxo-dG Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Skin tissue
    Species: Human
    Verified Customer | Posted 12/28/2017
    8-oxo-dG Antibody (15A3) 4354-MC-050

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