Aminopeptidase P1/XPNPEP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80613

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Aminopeptidase P1/XPNPEP1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DGAFGIRIENVVLVVPVKTKYNFNNRGSLTFEPLTLVPIQTKMIDVDSLTDKECDWLNNYHLTCRDVIGKELQKQGRQEALEWLIRETQPISKQH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aminopeptidase P1/XPNPEP1 Antibody - BSA Free

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80613]

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80613]

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80613] - Analysis using Anti-XPNPEP1 antibody NBP1-80613 (A) shows similar pattern to independent antibody NBP1-80614 (B).
Immunocytochemistry/ Immunofluorescence: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80613]

Immunocytochemistry/ Immunofluorescence: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80613]

Immunocytochemistry/Immunofluorescence: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80613] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80613]

Immunohistochemistry-Paraffin: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80613]

Immunohistochemistry-Paraffin: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80613] - Staining of human duodenum shows cytoplasmic positivity in glandular cells.
Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80613]

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80613]

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80613] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80613]

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80613]

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80613] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Applications for Aminopeptidase P1/XPNPEP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aminopeptidase P1/XPNPEP1

XPNPEP1 is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of any unsubstituted N-terminal amino acid that is adjacent to a penultimate proline residue. As a result of its specificity toward proline, it has been suggested that XPNPEP1 is important in the maturation and degradation of peptide hormones, neuropeptides, and tachykinins, as well as in the digestion of otherwise resistant dietary protein fragments, thereby complementing the pancreatic peptidases. Deficiency of XPNPEP1 results in excretion of large amounts of imino-oligopeptides in urine.

Alternate Names

APP1, SAMP, XPNPEP1

Entrez Gene IDs

7511 (Human)

Gene Symbol

XPNPEP1

UniProt

Q9NQW7

Additional Aminopeptidase P1/XPNPEP1 Products

Product Documents for Aminopeptidase P1/XPNPEP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Aminopeptidase P1/XPNPEP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Metalloproteases and Regulators

Citations for Aminopeptidase P1/XPNPEP1 Antibody - BSA Free

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Protocols

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