B7-H3/CD276 Antibody (MJ18) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-75195

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # MJ18

Format

Azide and BSA Free
View all B7-H3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated by immunizing Sprague Dawley rats with B7-H3-Ig, consisting of the extracellular domain (aa1-242 of mouse B7-H3) linked to the Fc portion of mouse IgG2a, emulsified in CFA (Difco). The antibody was selected by its reactivity to mouse B7-H3-transfected cells, but not to parental cells by flow cytometry.

Specificity

This antibody binds to mouse B7-H3 and does not react with other B7-family molecules.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for B7-H3/CD276 Antibody (MJ18) - Chimeric - Azide and BSA Free

Flow Cytometry: B7-H3/CD276 Antibody (MJ18) - Chimeric - Azide and BSA Free [NBP2-75195]

Flow Cytometry: B7-H3/CD276 Antibody (MJ18) - Chimeric - Azide and BSA Free [NBP2-75195]

Flow Cytometry: B7-H3/CD276 Antibody (MJ18) - Chimeric [NBP2-75195] - Immunofluorescence staining of fixed mouse splenocytes with anti-B7-H3 (CD276) antibody MJ18. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of MJ18 (NBP2-75195) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing weak membrane staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75195, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody
ELISA: B7-H3/CD276 Antibody (MJ18) - Chimeric - Azide and BSA Free [NBP2-75195]

ELISA: B7-H3/CD276 Antibody (MJ18) - Chimeric - Azide and BSA Free [NBP2-75195]

ELISA: B7-H3/CD276 Antibody (MJ18) - Chimeric [NBP2-75195] - Binding curve of anti-B7-H3 antibody MJ18 (NBP2-75195) to mouse B7-H3-Fc fusion protein ELISA Plate coated with mouse B7-H3-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP2-75195. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.

Applications for B7-H3/CD276 Antibody (MJ18) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100 - 1:1000
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: B7-H3

Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).

Long Name

B7 Homolog 3

Alternate Names

B7H3, CD276

Gene Symbol

CD276

Additional B7-H3 Products

Product Documents for B7-H3/CD276 Antibody (MJ18) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for B7-H3/CD276 Antibody (MJ18) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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