CD21 Antibody (CL14552) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44288

Novus Biologicals

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL14552

Format

Azide and BSA Free
View all CD21 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P20023, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse CD21 Antibody (CL14552) - Azide and BSA Free (NBP3-26866) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD21 Antibody (CL14552) - Azide and BSA Free

CD21 Antibody (CL14552) - Azide and BSA Free Immunohistochemistry-Paraffin: CD21 Antibody [NBP3-44288]

Immunohistochemistry-Paraffin: CD21 Antibody [NBP3-44288]

Analysis in human tonsil and skeletal muscle tissues using NBP3-44288 antibody. Corresponding CD21 RNA-seq data are presented for the same tissues.
CD21 Antibody (CL14552) - Azide and BSA Free Immunohistochemistry-Paraffin: CD21 Antibody [NBP3-44288]

Immunohistochemistry-Paraffin: CD21 Antibody [NBP3-44288]

Staining of human lymph node shows strong membranous positivity in germinal center cells.
CD21 Antibody (CL14552) - Azide and BSA Free Immunohistochemistry-Paraffin: CD21 Antibody [NBP3-44288]

Immunohistochemistry-Paraffin: CD21 Antibody [NBP3-44288]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
CD21 Antibody (CL14552) - Azide and BSA Free Immunohistochemistry-Paraffin: CD21 Antibody [NBP3-44288]

Immunohistochemistry-Paraffin: CD21 Antibody [NBP3-44288]

Staining of human small intestine shows strong membranous positivity in a subset of lymphoid cells.
CD21 Antibody (CL14552) - Azide and BSA Free Western Blot: CD21 Antibody [NBP3-44288]

Western Blot: CD21 Antibody [NBP3-44288]

Analysis in human cell line Daudi.
CD21 Antibody (CL14552) - Azide and BSA Free Immunohistochemistry-Paraffin: CD21 Antibody [NBP3-44288]

Immunohistochemistry-Paraffin: CD21 Antibody [NBP3-44288]

Staining of human tonsil shows strong membranous positivity in germinal center cells.

Applications for CD21 Antibody (CL14552) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD21

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.

Alternate Names

C3DR, CD21, CR2

Gene Symbol

CR2

Additional CD21 Products

Product Documents for CD21 Antibody (CL14552) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD21 Antibody (CL14552) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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