CD68/SR-D1 Antibody (CL1346) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44491

Novus Biologicals

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1346

Format

Azide and BSA Free
View all CD68/SR-D1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P34810, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse CD68/SR-D1 Antibody (CL1346) - Azide and BSA Free (NBP2-34482) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD68/SR-D1 Antibody (CL1346) - Azide and BSA Free

CD68/SR-D1 Antibody (CL1346) - Azide and BSA Free Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody [NBP3-44491]

Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody [NBP3-44491]

Analysis in human lung and skeletal muscle tissues using NBP3-44491 antibody. Corresponding CD68/SR-D1 RNA-seq data are presented for the same tissues.
CD68/SR-D1 Antibody (CL1346) - Azide and BSA Free Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody [NBP3-44491]

Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody [NBP3-44491]

Staining of human colon shows strong cytoplasmic positivity in lymphoid cells.
CD68/SR-D1 Antibody (CL1346) - Azide and BSA Free Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody [NBP3-44491]

Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody [NBP3-44491]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
CD68/SR-D1 Antibody (CL1346) - Azide and BSA Free Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody [NBP3-44491]

Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody [NBP3-44491]

Staining of human lung shows strong cytoplasmic positivity in macrophages.
CD68/SR-D1 Antibody (CL1346) - Azide and BSA Free Western Blot: CD68/SR-D1 Antibody [NBP3-44491]

Western Blot: CD68/SR-D1 Antibody [NBP3-44491]

Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1, using Anti-CD68/SR-D1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
CD68/SR-D1 Antibody (CL1346) - Azide and BSA Free Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody [NBP3-44491]

Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody [NBP3-44491]

Staining of human tonsil shows strong cytoplasmic positivity in lymphoid cells.

Applications for CD68/SR-D1 Antibody (CL1346) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD68/SR-D1

Human CD68, also known as GP110, LAMP4, Scavenger Receptor D1 (SR-D1) or macrosialin in mouse, encodes a 110-kD transmembrane glycoprotein that belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. Members of the LAMP family include LAMP-1, LAMP-2, dendritic cell (DC)-LAMP (aka CD208), and brain and dendritic cell-associated (BAD)-LAMP (aka LAMP-5). Unlike the two LAMP domains facing the lysosomal lumen in LAMP-1 and LAMP-2, CD68 has a single LAMP domain containing four cystines spaced 36-37 residues apart along with an N-terminal Mucin-like domain. The 354 amino acid (a.a.) human CD68 and 326 a.a. murine ortholog share 80.6% a.a. sequence identity (1).

CD68 is highly expressed in cells of the mononuclear phagocyte system such as macrophages, microglia, osteoclasts, and myeloid dendritic cells (DCs); and is expressed to a lesser extent in lymphoid cells (CD19+ B lymphocytes and CD4+ T lymphocytes), human umbilical cord mesenchymal stem cells (MSCs), fibroblasts, endothelial cells, multiple non-hematopoietic cancer cell lines, and human arterial intimal smooth muscle cells (SMCs). Expression has been also observed in diseased states for granulocytes and neutrophils, in particular basophils from myeloproliferative disorders and intestinal neutrophils from inflammatory bowel disease (IBD), respectively (1).

Although the function of CD68 has yet to be established, it has often been used as an immunohistochemistry (IHC) marker of inflammation and for granular cell tumors (GCTs). CD68+ tumor associated macrophages (TAMs) has been suggested to be a predictive marker for poor cancer prognosis, but a meta-analysis showed the presence of CD68 is not correlated with survival (2). In addition, a role in hepatic malaria infection has been reported based on the finding that peptide P39 binds CD68, considered a receptor for malaria sporozoite, and inhibits parasite entry into Kupffer cells. CD68 was deemed a member of the Scavenger receptor family due to its upregulation in macrophages following inflammatory stimuli, ability to bind modified LDL, phosphatidylserine, and apoptotic cells, as well as shuttling between the plasma membrane and endosomes. CD68 has been linked to atherogenesis based on binding and internalization of its ligand, oxLDL (1).

References

1. Chistiakov, DA, Killingsworth, MC, Myasoedova, VA. Orekhov AN, Bobryshev YV. (2017) CD68/macrosialin: not just a histochemical marker. Lab Invest. 97:4-13. PMID: 27869795

2. Troiano G, Caponio VCA, Adipietro I, Tepedino M, Santoro R, Laino L, Lo Russo L, Cirillo N, Lo Muzio L. (2019) Prognostic significance of CD68+ and CD163+ tumor associated macrophages in head and neck squamous cell carcinoma: A systematic review and meta-analysis. Oral Oncol. 93:66-75. PMID: 31109698.

Alternate Names

CD68, gp110, Macrosialin, SCARD1, SR-D1, SRD1

Gene Symbol

CD68

Additional CD68/SR-D1 Products

Product Documents for CD68/SR-D1 Antibody (CL1346) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD68/SR-D1 Antibody (CL1346) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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