CDK4 Antibody (4F11) - Azide and BSA Free

Novus Biologicals | Catalog # H00001019-M03

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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4F11

Format

Azide and BSA Free
View all CDK4 Primary Antibodies »

Product Specifications

Immunogen

CDK4 (AAH03644, 211 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

CDK4 (4F11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CDK4 Antibody (4F11) - Azide and BSA Free

Western Blot: CDK4 Antibody (4F11) [H00001019-M03]

Western Blot: CDK4 Antibody (4F11) [H00001019-M03]

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody (M03), clone 4F11. Lane 1: CDK4 transfected lysatE (33.7 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: CDK4 Antibody (4F11) [H00001019-M03]

Immunocytochemistry/ Immunofluorescence: CDK4 Antibody (4F11) [H00001019-M03]

Immunocytochemistry/Immunofluorescence: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of monoclonal antibody to CDK4 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: CDK4 Antibody (4F11) [H00001019-M03]

Western Blot: CDK4 Antibody (4F11) [H00001019-M03]

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western Blot analysis of CDK4 expression in HeLa ( Cat # L013V1 ).
Western Blot: CDK4 Antibody (4F11) [H00001019-M03]

Western Blot: CDK4 Antibody (4F11) [H00001019-M03]

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western Blot analysis of CDK4 expression in Raw 264.7 ( Cat # L024V1 ).
Western Blot: CDK4 Antibody (4F11) [H00001019-M03]

Western Blot: CDK4 Antibody (4F11) [H00001019-M03]

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western Blot analysis of CDK4 expression in NIH/3T3 ( Cat # L018V1 ).
Western Blot: CDK4 Antibody (4F11) [H00001019-M03]

Western Blot: CDK4 Antibody (4F11) [H00001019-M03]

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western blot analysis of CDK4 over-expressed 293 cell line. Lane 2 cotransfected with CDK4 Validated Chimera RNAi or non-transfected control. Blot probed with H00001019-M03. GAPDH (36.1 kDa) used as loading control.
Western Blot: CDK4 Antibody (4F11) [H00001019-M03]

Western Blot: CDK4 Antibody (4F11) [H00001019-M03]

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in 293.
Western Blot: CDK4 Antibody (4F11) [H00001019-M03]

Western Blot: CDK4 Antibody (4F11) [H00001019-M03]

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in Hela S3 NE.
ELISA: CDK4 Antibody (4F11) [H00001019-M03]

ELISA: CDK4 Antibody (4F11) [H00001019-M03]

ELISA: CDK4 Antibody (4F11) [H00001019-M03] - Detection limit for recombinant GST tagged CDK4 is approximately 1ng/ml as a capture antibody.

Applications for CDK4 Antibody (4F11) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CDK4

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Long Name

Cyclin-dependent Kinase 4

Alternate Names

CMM3, PSK-J3

Entrez Gene IDs

1019 (Human)

Gene Symbol

CDK4

OMIM

123829 (Human)

UniProt

AAH03644 (Human)

Additional CDK4 Products

Product Documents for CDK4 Antibody (4F11) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CDK4 Antibody (4F11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Intracellular Kinases

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Protocols

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FAQs

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