CEACAM-3 (carcinoembryonic antigen-related cell adhesion molecule 3; also CD66d and CGM1) is a 31 - 34 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and serves as a microbial receptor for a wide range of microorganisms. Mature human CEACAM-3 is a 218 amino acid (aa) type I transmembrane glycoprotein. Its extracellular domain (aa 35 - 155) shows one V-type Ig-like domain (aa 35 - 142). There are two alternate splice forms of CEACAM-3. One shows a 40 aa substitution for the C-terminal 116 amino acids (aa 137 - 252), while a second shows a 31 aa substitution for the C-terminal 71 amino acids. No definitive rodent CEACAM-3 has been reported.
CEACAM3/CD66d Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-99909
![Immunohistochemistry-Paraffin: CEACAM3/CD66d Antibody [NBP2-99909]](https://resources.rndsystems.com/images/products/CEACAM3-CD66d-Antibody-Immunohistochemistry-Paraffin-NBP2-99909-img0001.jpg "Immunohistochemistry-Paraffin: CEACAM3/CD66d Antibody [NBP2-99909]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human CEACAM3/CD66d (Accession#: NP_001806.2; Met1-Gly155)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for CEACAM3/CD66d Antibody - Azide and BSA Free
Immunohistochemistry-Paraffin: CEACAM3/CD66d Antibody [NBP2-99909]
Immunohistochemistry-Paraffin: CEACAM3/CD66d Antibody [NBP2-99909] - Immunochemical staining of human CEACAM3/CD66d in human colon carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.Applications for CEACAM3/CD66d Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunohistochemistry-Paraffin
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CEACAM-3/CD66d
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 3
Alternate Names
CD66d, CGM1
Gene Symbol
CEACAM3
Additional CEACAM-3/CD66d Products
Product Documents for CEACAM3/CD66d Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CEACAM3/CD66d Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
