Cotton Rat CD8 alpha Antibody (JG12)

R&D Systems | Catalog # MAB7080

R&D Systems
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Key Product Details

Species Reactivity

Cotton Rat

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # JG12
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Product Specifications

Immunogen

NIH‑3T3 mouse embryonic fibroblast cell line transfected with cotton rat CD8 alpha and SP2/O mouse melanoma cell line transfected with cotton rat CD8 alpha.
Accession # AAL55392

Specificity

Detects cotton rat CD8 alpha in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Cotton Rat CD8 alpha Antibody (JG12)

Detection of CD8a antibody in Cotton Rat Splenocytes antibody by Flow Cytometry.

Detection of CD8 alpha in Cotton Rat Splenocytes by Flow Cytometry.

Cotton rat splenocytes were stained with Mouse Anti-Cotton Rat CD8a Monoclonal Antibody (Catalog # MAB7080, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Cotton Rat CD8 alpha Antibody (JG12)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Cotton rat splenocytes

Reviewed Applications

Read 1 review rated 1 using MAB7080 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD8

CD8 alpha (CD8 alpha ; also OX8, Leu-2 and Lyt-2) is a 23 kDa (predicted) member of the Ig superfamily of molecules. It is found on immature thymocytes, cytotoxic and suppressor T cells, select mast cells, and splenic and bone marrow dendritic cells (in rodent). On T cells, CD8 alpha exists as either a disulfide linked homodimer, or heterodimer bound to CD8 beta. CD8 alpha beta is best known as a coreceptor for the TCR, enhancing TCR signaling. CD8 alpha alpha serves a different function and acts as a TCR corepressor that blocks T cell activation. Based on rat, mature Sigmodon hispidus/cotton rat CD8 alpha is a 210 amino acid (aa) type I transmembrane protein that consists of a 161 aa extracellular region (aa 24-184) with one V-type Ig-like domain (aa 35-135), and a 30 aa cytoplasmic tail (aa 206-235). Although Sigmodon hispidus is called a rat, it is not. It is a rodent, and rat CD8 alpha is the closest ortholog to cotton rat CD8 alpha currently reported. Over the extracellular region, rat and cotton rat CD8 alpha share only 54% aa identity. Rat CD8 alpha runs at 34-39 kDa in SDS-Page, and possesses one utilized N-linked and O-linked glycosylation site (vs. no N-linked sites in cotton rat CD8 alpha ). Based on this organization, and assuming O-linked glycosylation, cotton rat CD8 alpha would be expected to run at about 30-34 kDa in SDS-PAGE. Over aa 24-182, cotton rat CD8 alpha shares 54% and 48% aa identity with rat and mouse CD8 alpha, respectively.   

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

UniProt

AAL55392 (Cotton Rat)

Additional CD8 Products

Product Documents for Cotton Rat CD8 alpha Antibody (JG12)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Cotton Rat CD8 alpha Antibody (JG12)

For research use only

Customer Reviews for Cotton Rat CD8 alpha Antibody (JG12) (1)

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  • Cotton Rat CD8 alpha Antibody
    Name: Magdalena Migalska
    Application: Flow Cytometry
    Sample Tested: Thymocytes
    Species: Bank vole (Myodes glareolus)
    Verified Customer | Posted 06/30/2020
    Bank vole thymocytes. Gating – lymphocytes. A- no staining. B- control staining – only 2nd-ry mAb. C- primary anti-Cotton Rat CD8a Antibody +2nd-ry mAb. D- Gating – all events, overlay: black – as in A, blue – as in B, green – as in C.
    Test of cross-reactivity of antibodies between closely related species – the cotton rat and the bank vole; Family: Cricetidae. Bank vole thymocytes were stained with Mouse Anti-Cotton Rat CD8a Monoclonal Antibody (Catalog # MAB7080), followed by FITC-conjugated Anti-Mouse IgG Secondary Antibody. Cell were analyzed with CytoFLEX (Beckman Coulter). The clone (JG12) does not cross-react with bank vole CD8.
    Cotton Rat CD8 alpha Antibody (JG12) MAB7080
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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