DC8 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-2575

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DC8 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 150 and 200 of human DC8 (C1orf48) using the numbering given in entry NP_056286.2 (GeneID 25936 ).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DC8 Antibody - BSA Free

Immunohistochemistry-Paraffin: DC8 Antibody [NB100-2575]

Immunohistochemistry-Paraffin: DC8 Antibody [NB100-2575]

Immunohistochemistry-Paraffin: DC8 Antibody [NB100-2575] - Detection of human DC8 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DC8 (NB100-2575) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: DC8 Antibody [NB100-2575]

Immunohistochemistry-Paraffin: DC8 Antibody [NB100-2575]

Immunohistochemistry-Paraffin: DC8 Antibody [NB100-2575] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-DC8 used at a dilution of 1:250.
Immunohistochemistry: DC8 Antibody [NB100-2575]

Immunohistochemistry: DC8 Antibody [NB100-2575]

Immunohistochemistry: DC8 Antibody [NB100-2575] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DC8 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry: DC8 Antibody [NB100-2575]

Immunohistochemistry: DC8 Antibody [NB100-2575]

Immunohistochemistry: DC8 Antibody [NB100-2575] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DC8 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: DC8 Antibody [NB100-2575]

Immunoprecipitation: DC8 Antibody [NB100-2575]

Immunoprecipitation: DC8 Antibody [NB100-2575] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-2575 used for IP at 3 mcg/mg lysate. DC8 was also immunoprecipitated by rabbit anti-DC8 antibodies NB100-2576.
DC8 Antibody [NB100-2575] - Detection of human DC8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti- DC8 antibody NB100-2575 used for IP at 6 ug per reaction. DC8 was also immunoprecipitated by another rabbit anti-DC8 antibody. For blotting immunoprecipitated DC8, NB100-2575 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for DC8 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DC8

This gene encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. It is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternate Names

C1orf48, chromosome 1 open reading frame 48, DC8, DKFZP566O1646, kinetochore-associated protein NSL1 homolog, MIS14, NSL1, MIND kinetochore complex component, homolog (S. cerevisiae)

Entrez Gene IDs

25936 (Human)

Gene Symbol

NSL1

UniProt

NP_056286.2 (Human)

Additional DC8 Products

Product Documents for DC8 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DC8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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