Deoxycytidylate deaminase Antibody (4B9) - Azide and BSA Free

Novus Biologicals | Catalog # H00001635-M01

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 4B9

Format

Azide and BSA Free
View all Deoxycytidylate deaminase Primary Antibodies »

Product Specifications

Immunogen

DCTD (NP_001912.2, 69 a.a. ~ 178 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RTAENKLDTKYPYVCHAELNAIMNKNSTDVKGCSMYVALFPCNECAKLIIQAGIKEVIFMSDKYHDSDEATAARLLFNMAGVTFRKFIPKCSKIVIDFDSINSRPSQKLQ

Specificity

DCTD - dCMP deaminase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Deoxycytidylate deaminase Antibody (4B9) - Azide and BSA Free

Western Blot: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01]

Western Blot: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01]

Western Blot: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01] - Analysis of DCTD expression in transfected 293T cell line by DCTD monoclonal antibody (M01), clone 4B9.Lane 1: DCTD transfected lysate(20.016 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01]

Immunohistochemistry-Paraffin: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01]

Immunohistochemistry-Paraffin: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01] - Analysis of monoclonal antibody to DCTD on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
Western Blot: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01]

Western Blot: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01]

Western Blot: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01] - Analysis of DCTD over-expressed 293 cell line, cotransfected with DCTD Validated Chimera RNAi ( Cat # H00001635-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DCTD monoclonal antibody (M01), clone 4B9 (Cat # H00001635-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: Deoxycytidylate deaminase Antibody (4B9) [H00001635-M01] - Detection limit for recombinant GST tagged DCTD is 0.03 ng/ml as a capture antibody.

Applications for Deoxycytidylate deaminase Antibody (4B9) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for IHC-P, ELISA and RNAi Validation.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Deoxycytidylate deaminase

The protein encoded by this gene catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The encoded protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. This protein uses zinc as a cofactor for its activity. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

dCMP deaminaseEC 3.5.4.12, deoxycytidylate deaminase, MGC111062

Entrez Gene IDs

1635 (Human)

Gene Symbol

DCTD

OMIM

607638 (Human)

UniProt

NP_001912 (Human)

Additional Deoxycytidylate deaminase Products

Product Documents for Deoxycytidylate deaminase Antibody (4B9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Deoxycytidylate deaminase Antibody (4B9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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