DHX33 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-2580

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DHX33 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 33 using the numbering given in entry NP_064547.2 (GeneID 56919).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DHX33 Antibody - BSA Free

Immunohistochemistry: DHX33 Antibody [NB100-2580]

Immunohistochemistry: DHX33 Antibody [NB100-2580]

Immunohistochemistry: DHX33 Antibody [NB100-2580] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DHX33 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Counterstain: IHC Hematoxylin (blue).
Immunohistochemistry: DHX33 Antibody [NB100-2580]

Immunohistochemistry: DHX33 Antibody [NB100-2580]

Immunohistochemistry: DHX33 Antibody [NB100-2580] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:250. Detection by DAB staining. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH.
Immunohistochemistry: DHX33 Antibody [NB100-2580]

Immunohistochemistry: DHX33 Antibody [NB100-2580]

Immunohistochemistry: DHX33 Antibody [NB100-2580] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH.
Immunohistochemistry-Paraffin: DHX33 Antibody [NB100-2580]

Immunohistochemistry-Paraffin: DHX33 Antibody [NB100-2580]

Immunohistochemistry-Paraffin: DHX33 Antibody [NB100-2580] - Human lung carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).
Immunohistochemistry: DHX33 Antibody [NB100-2580]

Immunohistochemistry: DHX33 Antibody [NB100-2580]

Immunohistochemistry: DHX33 Antibody [NB100-2580] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DHX33 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Counterstain: IHC Hematoxylin (blue).
Immunoprecipitation: DHX33 Antibody [NB100-2580]

Immunoprecipitation: DHX33 Antibody [NB100-2580]

Immunoprecipitation: DHX33 Antibody [NB100-2580] - Detection of Human DHX33 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DHX33 antibody NB100-2580 used for IP at 3 mcg/mg lysate. DHX33 was also immunoprecipitated by rabbit anti-DHX33 antibody NB100-2581. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for DHX33 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DHX33

DHX33 is a DEAD box protein whose function has not yet been determined. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Alternate Names

DDX33, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 33, DEAH (Asp-Glu-Ala-His) box polypeptide 33, DEAH box protein 33, DKFZp762F2011, EC 3.6.1, EC 3.6.4.13, FLJ21972, putative ATP-dependent RNA helicase DHX33

Entrez Gene IDs

56919 (Human)

Gene Symbol

DHX33

UniProt

Q9H6R0 (Human)

Additional DHX33 Products

Product Documents for DHX33 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DHX33 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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