E6AP/UBE3A Antibody (2F6) - Azide and BSA Free

Novus Biologicals | Catalog # H00007337-M01

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F6

Format

Azide and BSA Free
View all E6AP/UBE3A Primary Antibodies »

Product Specifications

Immunogen

UBE3A (AAH09271, 51 a.a. ~ 150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGEVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPRVDPLETELGVKTLDCR

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33737669).

Specificity

UBE3A - ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for E6AP/UBE3A Antibody (2F6) - Azide and BSA Free

Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01]

Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01]

Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - UBE3A monoclonal antibody (M01), clone 2F6. Analysis of UBE3A expression in PC-12.
Immunocytochemistry/ Immunofluorescence: E6AP/UBE3A Antibody (2F6) [H00007337-M01]

Immunocytochemistry/ Immunofluorescence: E6AP/UBE3A Antibody (2F6) [H00007337-M01]

Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Analysis of monoclonal antibody to UBE3A on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody (2F6) [H00007337-M01]

Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody (2F6) [H00007337-M01]

Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Analysis of monoclonal antibody to UBE3A on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.
Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01]

Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01]

Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - UBE3A expression in Jurkat ( Cat # L017V1 ).
ELISA: E6AP/UBE3A Antibody (2F6) [H00007337-M01]

ELISA: E6AP/UBE3A Antibody (2F6) [H00007337-M01]

ELISA: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Detection limit for recombinant GST tagged UBE3A is approximately 0.03ng/ml as a capture antibody.
E6AP/UBE3A Antibody (2F6)

Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] -

Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Effect of E6AP silencing on the stability of HPV E6. (A)E6AP knockdown was confirmed by immunoblotting. (B) HEK293T cells (E6AP-null) were transfected with HPV16 E6 or HPV18 E6. (C) After 48 h the cells were harvested, & protein levels analyzed by immunoblotting. beta -actin acted as a control for transfection efficiency. (D) HEK293T cells (E6AP-null) were transfected with the indicated expression plasmids; after 36 h they were treated with 10 μM MG132 & 12 h later, harvested & subjected to immunoprecipitation (IP) with anti-myc-conjugated agarose beads. Polyubiquitinated HPV E6 was then detected for IP with an antibody against HA. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31749782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for E6AP/UBE3A Antibody (2F6) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Use in IHC-Fr reported in scientific literature (PMID:33737669). Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: E6AP/UBE3A

This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

Long Name

Human Papillomavirus E6-associated Protein

Alternate Names

ANCR, EPVE6AP, HPVE6A, UBE3A

Entrez Gene IDs

7337 (Human)

Gene Symbol

UBE3A

OMIM

105830 (Human)

UniProt

AAH09271 (Human)

Additional E6AP/UBE3A Products

Product Documents for E6AP/UBE3A Antibody (2F6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for E6AP/UBE3A Antibody (2F6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases

Citations for E6AP/UBE3A Antibody (2F6) - Azide and BSA Free

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Protocols

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway
Ubiquitination Cascade Pathway Thumbnail