FEN-1 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-321
![Western Blot: FEN-1 Antibody [NB100-321]](https://resources.rndsystems.com/images/products/FEN-1-Antibody-Western-Blot-NB100-321-img0012.jpg "Western Blot: FEN-1 Antibody [NB100-321]")
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Sheep (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human Flap Structure Endonuclease 1 using the numbering given in entry NP_004102.1 (GeneID 2237).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FEN-1 Antibody - BSA Free
Western Blot: FEN-1 Antibody [NB100-321]
Western Blot: FEN-1 Antibody [NB100-321] - Detection of Human Fen1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody NB100-321 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunohistochemistry-Paraffin: FEN-1 Antibody [NB100-321]](https://resources.rndsystems.com/images/products/FEN-1-Antibody-Immunohistochemistry-NB100-321-img0013.jpg "Immunohistochemistry-Paraffin: FEN-1 Antibody [NB100-321]")
Immunohistochemistry-Paraffin: FEN-1 Antibody [NB100-321]
Immunohistochemistry-Paraffin: FEN-1 Antibody [NB100-321] - FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Fen1 (NB100-321) used at a dilution of 1:1000 (1ug/ml). Detection: DAB![Immunoprecipitation: FEN-1 Antibody [NB100-321]](https://resources.rndsystems.com/images/products/FEN-1-Antibody-Immunoprecipitation-NB100-321-img0014.jpg "Immunoprecipitation: FEN-1 Antibody [NB100-321]")
Immunoprecipitation: FEN-1 Antibody [NB100-321]
Immunoprecipitation: FEN-1 Antibody [NB100-321] - Detection of human Fen1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody NB100-321 (lot NB100-321-2) used for IP at 6 ug per reaction. Fen1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-321-1). For blotting immunoprecipitated Fen1, NB100-321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for FEN-1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:200- 1:1000
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. 16-18 hour incubation is recommended
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: FEN-1
Long Name
Flap Endonuclease 1
Alternate Names
FEN1
Entrez Gene IDs
2237 (Human)
Gene Symbol
FEN1
UniProt
Additional FEN-1 Products
Product Documents for FEN-1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FEN-1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for FEN-1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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