Fibronectin Antibody (568) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-48338

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 568

Format

IHC-Prediluted
View all Fibronectin Primary Antibodies »

Product Specifications

Immunogen

High molecular weight proteins secreted by cultivated human fibroblasts (Uniprot: P02751)

Reactivity Notes

Use in Human reported in scientific literature (PMID:34474855)

Localization

Connective tissue matrix

Specificity

Epitope of this monoclonal antibody is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This monoclonal antibody is excellent for staining of formalin-fixed, paraffin-embedded tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Fibronectin Antibody (568) - IHC-Prediluted

Fibronectin Antibody (568) - IHC-Prediluted Immunohistochemistry-Paraffin: Fibronectin Antibody (568) - IHC-Prediluted [NBP2-48338] -

Immunohistochemistry-Paraffin: Fibronectin Antibody (568) - IHC-Prediluted [NBP2-48338] -

Immunohistochemistry-Paraffin: Fibronectin Antibody (568) - IHC-Prediluted [NBP2-48338] - Human Renal Cell Carcinoma stained with Fibronectin Monoclonal Antibody (568).
Fibronectin Antibody (568) - IHC-Prediluted Western Blot: Fibronectin Antibody (568) - IHC-Prediluted [NBP2-48338] -

Western Blot: Fibronectin Antibody (568) - IHC-Prediluted [NBP2-48338] -

Western Blot Analysis of Human Placenta tissue lysate using Fibronectin Antibody (568) - IHC-Prediluted.
Fibronectin Antibody (568) - IHC-Prediluted Western Blot: Fibronectin Antibody (568) - IHC-Prediluted [NBP2-48338] -

Western Blot: Fibronectin Antibody (568) - IHC-Prediluted [NBP2-48338] -

Western Blot Analysis of Human Placenta tissue lysate using Fibronectin Antibody (568) - IHC-Prediluted.

Applications for Fibronectin Antibody (568) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Fibronectin

Fibronectin (also known as FN1, or Fibronectin 1) is an extracellular matrix glycoprotein that binds to integrins and has a large role in cell adhesion, migration, differentiation, and growth. It plays a key role in wound healing by helping form blood clots and participates in embryonic development by guiding cell attachment and migration. Fibronectin consists of two monomers linked by disulfide bonds and binds extracellular components such as collagen fibrin and syndecans. Fibronectin has been implicated in carcinoma and tumor development while also limiting tumor cells response to treatment. Novus offers Fibronectin antibodies, ELISA Kits, lysates, and proteins

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Gene Symbol

FN1

Additional Fibronectin Products

Product Documents for Fibronectin Antibody (568) - IHC-Prediluted

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Fibronectin Antibody (568) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Fibronectin Antibody (568) - IHC-Prediluted

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    • Q: What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?
      A: Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application. 
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