Fibronectin Antibody (CL3730) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-43942
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL3730
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P02751, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse Fibronectin Antibody (CL3730) - Azide and BSA Free (NBP2-61633) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Fibronectin Antibody (CL3730) - Azide and BSA Free
![Fibronectin Antibody (CL3730) - Azide and BSA Free Western Blot: Fibronectin Antibody [NBP3-43942]](https://beta-resources.rndsystems.com/images/products/nbp3-43942_mouse-fibronectin-mab-cl3730-azide-and-bsa-free-2822025127377.jpg "Western Blot: Fibronectin Antibody [NBP3-43942]")
Western Blot: Fibronectin Antibody [NBP3-43942]
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line U-87 MG
![Fibronectin Antibody (CL3730) - Azide and BSA Free Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]](https://beta-resources.rndsystems.com/images/products/nbp3-43942_mouse-fibronectin-mab-cl3730-azide-and-bsa-free-28220251273789.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]")
Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]
Staining of human breast cancer shows strong immunoreactivity in tumor stroma.![Fibronectin Antibody (CL3730) - Azide and BSA Free Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]](https://beta-resources.rndsystems.com/images/products/nbp3-43942_mouse-fibronectin-mab-cl3730-azide-and-bsa-free-282202512324837.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]")
Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]
Staining of human liver cancer shows strong positivity in tumor.![Fibronectin Antibody (CL3730) - Azide and BSA Free Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]](https://beta-resources.rndsystems.com/images/products/nbp3-43942_mouse-fibronectin-mab-cl3730-azide-and-bsa-free-282202512303882.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]")
Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]
Staining of human endometrium shows strong immunoreactivity in endometrial stroma and basement membrane of glandular cells.![Fibronectin Antibody (CL3730) - Azide and BSA Free Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]](https://beta-resources.rndsystems.com/images/products/nbp3-43942_mouse-fibronectin-mab-cl3730-azide-and-bsa-free-282202512495872.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]")
Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]
Staining of human prostate shows moderate positivity in smooth muscle.![Fibronectin Antibody (CL3730) - Azide and BSA Free Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]](https://beta-resources.rndsystems.com/images/products/nbp3-43942_mouse-fibronectin-mab-cl3730-azide-and-bsa-free-28220251344257.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]")
Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]
Staining of human placenta shows strong immunoreactivity in chorionic villi.![Fibronectin Antibody (CL3730) - Azide and BSA Free Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]](https://beta-resources.rndsystems.com/images/products/nbp3-43942_mouse-fibronectin-mab-cl3730-azide-and-bsa-free-2822025135323.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]")
Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]
Staining of human pancreas shows absence of immunoreactivity in glandular cells (negative control).![Fibronectin Antibody (CL3730) - Azide and BSA Free Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]](https://beta-resources.rndsystems.com/images/products/nbp3-43942_mouse-fibronectin-mab-cl3730-azide-and-bsa-free-282202513332364.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]")
Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]
Staining of human testis shows immunoreactivity in the connective tissue surrounding seminiferous tubules.![Fibronectin Antibody (CL3730) - Azide and BSA Free Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]](https://beta-resources.rndsystems.com/images/products/nbp3-43942_mouse-fibronectin-mab-cl3730-azide-and-bsa-free-282202513332320.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]")
Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]
Staining of human small intestine shows strong immunoreactivity in the connective tissue.Applications for Fibronectin Antibody (CL3730) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1 ug/ml
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: Fibronectin
Alternate Names
CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF
Gene Symbol
FN1
Additional Fibronectin Products
Product Documents for Fibronectin Antibody (CL3730) - Azide and BSA Free
Product Specific Notices for Fibronectin Antibody (CL3730) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Fibronectin Antibody (CL3730) - Azide and BSA Free
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Q: What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?
A: Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application.
