Fibronectin Antibody (OTI5C1) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-70762
![Western Blot: Fibronectin Antibody (OTI5C1)Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Western-Blot-NBP2-70762-img0001.jpg "Western Blot: Fibronectin Antibody (OTI5C1)Azide and BSA Free [NBP2-70762]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI5C1
Format
Azide and BSA Free
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 32-307 of human FN1 (NP_473375) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse Fibronectin Antibody (OTI5C1) - Azide and BSA Free (NBP2-45690) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Fibronectin Antibody (OTI5C1) - Azide and BSA Free
Western Blot: Fibronectin Antibody (OTI5C1)Azide and BSA Free [NBP2-70762]
Western Blot: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fibronectin.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0024.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Human thyroid tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0002.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Adenocarcinoma of Human breast tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0003.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Adenocarcinoma of Human colon tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0004.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Adenocarcinoma of Human endometrium tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0005.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Adenocarcinoma of Human ovary tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0006.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Carcinoma of Human bladder tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0007.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Carcinoma of Human kidney tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0008.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Carcinoma of Human liver tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0009.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Carcinoma of Human lung tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0010.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Carcinoma of Human pancreas tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0011.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Carcinoma of Human prostate tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0012.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Carcinoma of Human thyroid tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0013.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Human bladder tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0014.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Human breast tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0015.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Human colon tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0016.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Human endometrium tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0017.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Human Kidney tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0018.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Human lung tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0019.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Human lymph node tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0020.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Human lymphoma tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0021.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Human Ovary tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0022.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Human pancreas tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0023.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Human prostate tissue.![Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-OTI5C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70762-img0025.jpg "Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]")
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762]
Immunohistochemistry: Fibronectin Antibody (OTI5C1) - Azide and BSA Free [NBP2-70762] - Analysis of Human tonsil tissue.Applications for Fibronectin Antibody (OTI5C1) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: Fibronectin
Alternate Names
CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF
Gene Symbol
FN1
Additional Fibronectin Products
Product Documents for Fibronectin Antibody (OTI5C1) - Azide and BSA Free
Product Specific Notices for Fibronectin Antibody (OTI5C1) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Fibronectin Antibody (OTI5C1) - Azide and BSA Free
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Q: What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?
A: Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application.
