GATA-3 Antibody (CL8660) - BSA Free
Novus Biologicals | Catalog # NBP2-88917
![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]](https://resources.rndsystems.com/images/products/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0007.jpg "Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL8660
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed using a synthetic peptide derived from P23771, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for GATA-3 Antibody (CL8660) - BSA Free
![Western Blot: GATA-3 Antibody (CL8660) [NBP2-88917]](https://resources.rndsystems.com/images/products/GATA-3-Antibody-CL8660-Western-Blot-NBP2-88917-img0008.jpg "Western Blot: GATA-3 Antibody (CL8660) [NBP2-88917]")
Western Blot: GATA-3 Antibody (CL8660) [NBP2-88917]
Western Blot: GATA-3 Antibody (CL8660) [NBP2-88917] - Analysis in human cell line TD47D.![Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody (CL8660) [NBP2-88917]](https://resources.rndsystems.com/images/products/GATA-3-Antibody-CL8660-Immunocytochemistry-Immunofluorescence-NBP2-88917-img0001.jpg "Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody (CL8660) [NBP2-88917]")
Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody (CL8660) [NBP2-88917]
Immunocytochemistry/Immunofluorescence: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of MCF7 cells using the Anti-GATA3 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]](https://resources.rndsystems.com/images/products/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0002.jpg "Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]")
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]](https://resources.rndsystems.com/images/products/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0003.jpg "Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]")
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human kidney shows strong nuclear positivity in a small subset of cells in glomeruli.![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]](https://resources.rndsystems.com/images/products/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0004.jpg "Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]")
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]](https://resources.rndsystems.com/images/products/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0005.jpg "Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]")
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.![Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]](https://resources.rndsystems.com/images/products/GATA-3-Antibody-CL8660-Immunohistochemistry-Paraffin-NBP2-88917-img0006.jpg "Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]")
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.![GATA-3 Antibody (CL8660) - BSA Free Chromatin Immunoprecipitation-exo-Seq: GATA-3 Antibody (CL8660) - BSA Free [NBP2-88917]](https://resources.rndsystems.com/images/products/nbp2-88917_mouse-monoclonal-gata-3-antibody-cl8660-25620259103180.jpg "Chromatin Immunoprecipitation-exo-Seq: GATA-3 Antibody (CL8660) - BSA Free [NBP2-88917]")
Chromatin Immunoprecipitation-exo-Seq: GATA-3 Antibody (CL8660) - BSA Free [NBP2-88917]
ChIP-Exo-Seq composite graph for Anti-GATA3 (NBP2-88917) tested in MCF7 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for GATA-3 Antibody (CL8660) - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
1 ug/ml
Application Notes
For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GATA-3
Alternate Names
GATA3
Gene Symbol
GATA3
Additional GATA-3 Products
Product Documents for GATA-3 Antibody (CL8660) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GATA-3 Antibody (CL8660) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
