GDF-15 Antibody (CL0328) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-57860

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL0328

Format

Azide and BSA Free
View all GDF-15 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q99988, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GDF-15 Antibody (CL0328) - Azide and BSA Free

GDF-15 Antibody (CL0328) - BSA Free

GDF-15 Antibody (CL0328) - BSA Free

Analysis in human cell line RT-4.
GDF-15 Antibody (CL0328) - BSA Free

GDF-15 Antibody (CL0328) - BSA Free

Analysis in human placenta and skeletal muscle tissues using NBP3-57846 antibody. Corresponding GDF15 RNA-seq data are presented for the same tissues.
GDF-15 Antibody (CL0328) - BSA Free

GDF-15 Antibody (CL0328) - BSA Free

Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GDF15 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
GDF-15 Antibody (CL0328) - BSA Free

GDF-15 Antibody (CL0328) - BSA Free

Staining of human placenta shows moderate granular cytoplasmic positivity in trophoblastic cells.
GDF-15 Antibody (CL0328) - BSA Free

GDF-15 Antibody (CL0328) - BSA Free

Staining of human prostate shows strong granular cytoplasmic positivity in a subset of glandular cells.
GDF-15 Antibody (CL0328) - BSA Free

GDF-15 Antibody (CL0328) - BSA Free

Staining of human rectum shows strong cytoplasmic positivity in enteroendocrine cells.
GDF-15 Antibody (CL0328) - BSA Free

GDF-15 Antibody (CL0328) - BSA Free

Staining of human skeletal muscle shows no positivity in myocytes as expected.
GDF-15 Antibody (CL0328) - BSA Free

GDF-15 Antibody (CL0328) - BSA Free

Staining of human small intestine shows strong cytoplasmic positivity in enteroendocrine cells.

Applications for GDF-15 Antibody (CL0328) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDF-15

Growth differentiation factor 15 (GDF15) is a transforming growth factor beta superfamily member that acts to regulate inflammatory and apoptotic responses in injured or diseased tissues. GDF15 is most strongly expressed in the liver, placenta and kidney.

GDF15 antibodies are useful for studies on inflamation and immune system research.

Long Name

Growth Differentiation Factor 15

Alternate Names

GDF15, MIC-1, NAG-1, PDF, PLAB, PTGF-beta

Gene Symbol

GDF15

Additional GDF-15 Products

Product Documents for GDF-15 Antibody (CL0328) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GDF-15 Antibody (CL0328) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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