GLI-1 Antibody (HL247) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13667

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL247 expressed in HEK293

Format

Azide and BSA Free
View all GLI-1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse GLI-1. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for GLI-1 Antibody (HL247) - Azide and BSA Free

Western Blot: GLI-1 Antibody (HL247) [NBP3-13667]

Western Blot: GLI-1 Antibody (HL247) [NBP3-13667]

Western Blot: GLI-1 Antibody (HL247) [NBP3-13667] - Non-transfected (-) and transfected (+) RMS13 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GLI-1 antibody [HL247] (NBP3-13667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: GLI-1 Antibody (HL247) [NBP3-13667]

Immunohistochemistry-Paraffin: GLI-1 Antibody (HL247) [NBP3-13667]

Immunohistochemistry-Paraffin: GLI-1 Antibody (HL247) [NBP3-13667] - GLI-1 antibody [HL247] detects GLI-1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. GLI-1 stained by GLI-1 antibody [HL247] (NBP3-13667) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: GLI-1 Antibody (HL247) [NBP3-13667]

Western Blot: GLI-1 Antibody (HL247) [NBP3-13667]

Western Blot: GLI-1 Antibody (HL247) [NBP3-13667] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GLI-1 antibody [HL247] (NBP3-13667) diluted at 1:1000000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
GLI-1 Antibody (HL247)

Immunohistochemistry-Paraffin: GLI-1 Antibody (HL247) [NBP3-13667] -

Immunohistochemistry-Paraffin: GLI-1 Antibody (HL247) [NBP3-13667] - GLI-1 antibody [HL27] detects GLI-1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
GLI-1 stained by GLI-1 antibody [HL27] (NBP3-13667) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GLI-1 Antibody (HL247) - Azide and BSA Free

Western Blot: GLI-1 Antibody (HL247) [NBP3-13667] -

RMS 13 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (NBP3-13667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GLI-1 Antibody (HL247) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody (HL247) [NBP3-13667] -

Gli1 antibody [HL247] detects Gli1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Gli1 stained by Gli1 antibody [HL247] (NBP3-13667) diluted at 1:500.
Blue: Fluoroshield with DAPI.
GLI-1 Antibody (HL247) - Azide and BSA Free

Western Blot: GLI-1 Antibody (HL247) [NBP3-13667] -

Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL27] (NBP3-13667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for GLI-1 Antibody (HL247) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLI-1

Gli1 was produced against a peptide corresponding to the carboxy-terminal region of the mouse Gli-1 protein. This region is not conserved among other gli family members, namely Gli-2 and Gli-3. Gli was termed by Kinzler et al. (1987) as 'glioma-associated oncogene' amplified in malignant gliomas. Analysis of the cloned gene demonstrates that the gene contains 5 repeats of zinc-finger sequences, which places gli in the family of Kruppel (Kr) zinc finger proteins. Northern analysis reveals that GLI is expressed in embryonal carcinoma cells but not in most adult tissue. GLI has been localized to 12q13-q14.3 by Southern blot analysis. On mouse the gene is located on chromosome 10. In mice, 3 zinc finger transcription factors, Gli-1, Gl-i2 and Gli-3, have been implicated in the transduction of Sonic hedgehog (Shh) signal. In papillary epithelium, shh, gli1 and ptc all follow similar expression patterns. Gli1 expression is central and probably sufficient for tumor development in humans.

Long Name

Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]

Alternate Names

GLI1

Gene Symbol

GLI1

Additional GLI-1 Products

Product Documents for GLI-1 Antibody (HL247) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GLI-1 Antibody (HL247) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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