GLS2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76544

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GLS2 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 18 amino acid peptide near the center terminus of human GLS2 (NP_037399).

Reactivity Notes

Use in Human reported in scientific literature (PMID:33799686).

Specificity

Specificity for GLS2 versus GLS1 has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GLS2 Antibody - BSA Free

Western Blot: GLS2 AntibodyBSA Free [NBP1-76544]

Western Blot: GLS2 AntibodyBSA Free [NBP1-76544]

GLS2-Antibody-Western-Blot-NBP1-76544-img0009.jpg
Immunocytochemistry/ Immunofluorescence: GLS2 Antibody - BSA Free [NBP1-76544]

Immunocytochemistry/ Immunofluorescence: GLS2 Antibody - BSA Free [NBP1-76544]

Immunocytochemistry/Immunofluorescence: GLS2 Antibody [NBP1-76544] - of GLS2 in Human Kidney cells. Dilution 20 ug/mL.
Immunohistochemistry: GLS2 Antibody - BSA Free [NBP1-76544]

Immunohistochemistry: GLS2 Antibody - BSA Free [NBP1-76544]

Immunohistochemistry: GLS2 Antibody [NBP1-76544] - Immunohistochemistry of GLS2 in human kidney tissue with GLS2 antibody at 5 ug/mL.
Simple Western: GLS2 AntibodyBSA Free [NBP1-76544]

Simple Western: GLS2 AntibodyBSA Free [NBP1-76544]

Simple Western: GLS2 Antibody [NBP1-76544] - Simple Western lane view shows a specific band for GLS2 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
GLS2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GLS2 Antibody - BSA Free [NBP1-76544] -

Immunocytochemistry/ Immunofluorescence: GLS2 Antibody - BSA Free [NBP1-76544] - Histological analyses for assessing response to bevacizumab treatment. A Immunofluorescence staining of tumor vasculature & integrin beta 3 with antibodies against CD31 (green) & CD61 (integrin beta 3, red) in the treated & control groups at the end of the study. B Quantitative analysis of integrin beta 3 & CD31-positive area fraction of tumors between the control & treatment groups. **P < 0.05. Magnification: ×200. Microvascular density (CD31) from the therapy group (C) & control group (D). Tumor cell proliferation (Ki-67) from the therapy group (F) & control group (G). Quantitative MVD (E) & Ki67 (H) assessment of therapy & control group. Error bars denote standard errors, Mann–Whitney U-test were applied, **P < 0.05. Magnification: ×200 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31604441), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GLS2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Simple Western

1:100

Western Blot

0.5-1 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Hek293 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100, apparent MW was 60 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLS2

Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and alpha-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53's role in antioxidant defense in addition to its role in energy metabolism.

Alternate Names

EC 3.5.1.2, GAbreast cell glutaminase, GLSglutaminase GA, glutaminase 2 (liver, mitochondrial), glutaminase I, glutaminase liver isoform, mitochondrial, hLGA, LGA, L-glutaminase, L-glutamine amidohydrolase, MGC71567, phosphate-activated glutaminase, phosphate-dependent glutaminase, truncated glutaminase 2

Entrez Gene IDs

27165 (Human); 216456 (Mouse); 192268 (Rat)

Gene Symbol

GLS2

UniProt

Q9UI32 (Human)

Additional GLS2 Products

Product Documents for GLS2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GLS2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GLS2 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for GLS2 Antibody - BSA Free

Showing  1 - 2 的 2 FAQs Showing All

  • Q: I was wondering if you had any available references for GLS2 antibody?

    A: Unfortunately we are not aware of any references for this product at this time; however all of the species and applications listed on the datasheet are 100% guaranteed.

  • Q: What is the sequence which NBP1-76544 would recognize? It is described as "a 18 amino acid peptide near the center terminus of human GLS2" but may we ask if the specific sequence can be shared?

    A: The exact sequence of NBP1-76544 is proprietary, but it is from within amino acids 301-360 of NP_037399.

  • Q: I was wondering if you had any available references for GLS2 antibody?

    A: Unfortunately we are not aware of any references for this product at this time; however all of the species and applications listed on the datasheet are 100% guaranteed.

  • Q: What is the sequence which NBP1-76544 would recognize? It is described as "a 18 amino acid peptide near the center terminus of human GLS2" but may we ask if the specific sequence can be shared?

    A: The exact sequence of NBP1-76544 is proprietary, but it is from within amino acids 301-360 of NP_037399.

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