Glypican 4 Antibody (51E3) - BSA Free
Novus Biologicals | Catalog # NBP2-49892
![Western Blot: Glypican 4 Antibody (51E3)BSA Free [NBP2-49892]](https://resources.rndsystems.com/images/products/Glypican-4-Antibody-51E3-Western-Blot-NBP2-49892-img0008.jpg "Western Blot: Glypican 4 Antibody (51E3)BSA Free [NBP2-49892]")
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 51E3
Format
BSA Free
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Product Specifications
Immunogen
Recombinant human Glypican 4 (401-529aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Glypican 4 Antibody (51E3) - BSA Free
Western Blot: Glypican 4 Antibody (51E3)BSA Free [NBP2-49892]
Western Blot: Glypican 4 Antibody (51E3) [NBP2-49892] - Glypican 4 Antibody (AT51E3) [NBP2-49892] - The Recombinant Human GPC4 (50ng) and Cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPC4 anibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human GPC4 Lane 2. : Ramos cell lysate![Immunocytochemistry/ Immunofluorescence: Glypican 4 Antibody (51E3) - BSA Free [NBP2-49892]](https://resources.rndsystems.com/images/products/Glypican-4-Antibody-51E3-Immunocytochemistry-Immunofluorescence-NBP2-49892-img0009.jpg "Immunocytochemistry/ Immunofluorescence: Glypican 4 Antibody (51E3) - BSA Free [NBP2-49892]")
Immunocytochemistry/ Immunofluorescence: Glypican 4 Antibody (51E3) - BSA Free [NBP2-49892]
Immunocytochemistry/Immunofluorescence: Glypican 4 Antibody (51E3) [NBP2-49892] - Glypican 4 Antibody (AT51E3) [NBP2-49892] - analysis of GPC4 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GPC4 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).![Flow Cytometry: Glypican 4 Antibody (51E3) - BSA Free [NBP2-49892]](https://resources.rndsystems.com/images/products/Glypican-4-Antibody-51E3-Flow-Cytometry-NBP2-49892-img0005.jpg "Flow Cytometry: Glypican 4 Antibody (51E3) - BSA Free [NBP2-49892]")
Flow Cytometry: Glypican 4 Antibody (51E3) - BSA Free [NBP2-49892]
Flow Cytometry: Glypican 4 Antibody (51E3) [NBP2-49892] - Analysis of GPC4 in 293T cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).Applications for Glypican 4 Antibody (51E3) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:200
Immunocytochemistry/ Immunofluorescence
1:200
Western Blot
1:1000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Glypican 4
Alternate Names
GPC4, K-glypican
Gene Symbol
GPC4
Additional Glypican 4 Products
Product Documents for Glypican 4 Antibody (51E3) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Glypican 4 Antibody (51E3) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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