GPER/GPR30 Antibody - BSA Free

Novus Biologicals | Catalog # NLS4271

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human, Mouse, Rat

Predicted:

Canine (94%), Hamster (94%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GPER/GPR30 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 16 amino acid peptide from 3rd extracellular domain of human GPER/GPR30.

Epitope

3rd extracellular domain

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (100%), Bat (88%), Equine (88%), Rabbit (88%). Mouse reactivity reported in (PMID: 23273253). Rat reactivity reported from a verified customer review.

Localization

Endoplasmic reticulum - multi-pass membrane protein.

Specificity

Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for GPER/GPR30 Antibody - BSA Free

Western Blot: GPER/GPR30 AntibodyBSA Free [NLS4271]

Western Blot: GPER/GPR30 AntibodyBSA Free [NLS4271]

GPER-GPR30-Antibody-Western-Blot-NLS4271-img0004.jpg
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4271]

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody - BSA Free [NLS4271]

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4271] - Analysis of anti-GPR30 antibody with human brain, hippocampus.
Western Blot: GPER/GPR30 AntibodyBSA Free [NLS4271]

Western Blot: GPER/GPR30 AntibodyBSA Free [NLS4271]

Western Blot: GPER/GPR30 Antibody [NLS4271] - Analysis of anti-GPR30 antibody with rat hypothalamus 5-10ug protein loaded per lane. Image courtesy provided by Carrie McAllister.
GPER/GPR30 Antibody - BSA Free

Western Blot: GPER/GPR30 Antibody - BSA Free [NLS4271] -

Direct association of dopamine transporter and estrogen receptors on the plasma membrane vs. in total protein. Co-immunoprecipitation of dopamine transporter after 9 min 10-9 M E2 treatment and subsequent probing with antibodies for DAT, ER alpha, ER beta, and GPR30. This is a representative immunoblot of 3 experiments
GPER/GPR30 Antibody - BSA Free

Western Blot: GPER/GPR30 Antibody - BSA Free [NLS4271] -

Western Blot: GPER/GPR30 Antibody - BSA Free [NLS4271] - GPER/GPR30 expression in normal lung cell lines & lung adenocarcinoma cell lines. A) The expression of GPER was determined by realtime quantitative PCR in each of the normal lung cell lines (open bars), lung adenocarcinoma cell lines (grey bars), & MCF-7 human breast cancer cells (black bar). Values are the average of 4–6 biological replicates ± SEM. B-D) Representative western blot analysis of GPER/GPR30 expression in the indicted cell lines. 30 μg of whole cell extract (WCE) were separated on 10% SDS PAGE gels with MW marker migration indicated at the left of each blot. The diagrams at the top of B-C, & D indicate the region of GPER recognized by the 3 different polyclonal antibodies used: B) Novus NBP-1-31239, C) Novus NLS4271; D) Santa Cruz sc-4854-R. The MW of GPR30 is estimated to be 42 kDa, but higher MW sizes have been reported due to glycosylation & interaction with other proteins. Bands are identified as glycosylated & nonglycosylated based on reports cited in the text, but could include interaction with other proteins. For each GPER western, the membrane was stripped & reprobed for beta -actin as a loading control. Quantitation of GPER was evaluated by summing all immunoreactive bands & dividing by beta -actin, then normalizing to HBEC2-KT in each blot. Panel E is a summary of all westerns (not all westerns are shown) with each antibody. For Westerns with NBP1-31239 & sc-48524, values are the mean ± SEM from 3 separate blots using different WCE. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23273253), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GPER/GPR30 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

6.4 ug/ml

Western Blot

1:1000
Application Notes
Antibody does not work in Western Blot for mouse samples. IP usage is reported in scientific literature (PMID: 19531209). ELISA usage is reported in scientific literature (PMID: 18489713). Use in ICC/IF reported in scientific literature (PMID: 21427217)..

Reviewed Applications

Read 1 review rated 4 using NLS4271 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: GPER/GPR30

GPR30 is a transmembrane intracellular estrogen receptor (Revankar et al. 2005). Progestin inhibits growth in breast cancer cells through GPR30. GPR30 has been reported in brain, breast carcinoma, blood, bone marrow, CNS, heart, liver, lung, lymph node, placenta, and spleen. ESTs have been isolated from B-cell/lung/testis, brain, ganglion, heart/melanocyte/uterus, kidney, tonsil, liver/spleen, placenta, and prostate libraries. Ligands: Estrogen (Revankar et al. 2005). Caution: Rat GPR30 is also known as GPR41 and HIA-R.

Long Name

G Protein-coupled Estrogen Receptor 1

Alternate Names

CEPR, CMKRL2, DRY12, FEG-1, GPCR-BR, GPR30, LERGU2, LyGPR

Entrez Gene IDs

2852 (Human)

Gene Symbol

GPER1

UniProt

Q99527

Additional GPER/GPR30 Products

Product Documents for GPER/GPR30 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GPER/GPR30 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Obesity

Citations for GPER/GPR30 Antibody - BSA Free

Customer Reviews for GPER/GPR30 Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: rat hypothalamus
    Species: Rat
    Verified Customer | Posted 01/24/2013
    GPER/GPR30 Antibody - BSA Free NLS4271

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Protocols

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FAQs for GPER/GPR30 Antibody - BSA Free

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    • A: The expected molecular weight of GPR30 is 42.3, however, because this protein has at least 3 different glycosylation sites, you should not be surprised if you see a band at higher position in your samples.
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