HSF1 Antibody (4B4) - BSA Free

Novus Biologicals | Catalog # NBP2-42206

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, Rabbit

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Gel Super Shift Assays

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 4B4

Format

BSA Free
View all HSF1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Human, Rat, Bovine, Guinea Pig, Hamster, Monkey and Rabbit.

Localization

Cytoplasm, Nucleus

Specificity

Detects approx 85kDa (unstressed cell lysates) and approx 95kDa (heat shocked cell lysates).

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for HSF1 Antibody (4B4) - BSA Free

Western Blot: HSF1 Antibody (4B4) [NBP2-42206]

Western Blot: HSF1 Antibody (4B4) [NBP2-42206]

Western Blot: HSF1 Antibody (4B4) [NBP2-42206] - Western Blot analysis of Human Breast adenocarcinoma cell line (MCF7) showing detection of ~65 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (NBP2-42206). Lane 1: MW ladder. Lane 2: HSF1 null lysate prepared from mouse embryonic fibroblasts. Lane 3: MCF7 lysate (5 ug). Lane 4: MCF7 lysate (10 ug). Lane 5: MCF7 lysate (20 ug). Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42206) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rat IgG: HRP for 1 hour at RT. Predicted/Observed Size: ~65 kDa. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.
Immunocytochemistry/ Immunofluorescence: HSF1 Antibody (4B4) [NBP2-42206]

Immunocytochemistry/ Immunofluorescence: HSF1 Antibody (4B4) [NBP2-42206]

Immunocytochemistry/Immunofluorescence: HSF1 Antibody (4B4) [NBP2-42206] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x. Heat Shocked at 42C for 1h.
Western Blot: HSF1 Antibody (4B4) [NBP2-42206]

Western Blot: HSF1 Antibody (4B4) [NBP2-42206]

Western Blot: HSF1 Antibody (4B4) [NBP2-42206] - analysis of Human A431 and HEK293 cell lysates showing detection of HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:1000.
Immunocytochemistry/ Immunofluorescence: HSF1 Antibody (4B4) [NBP2-42206]

Immunocytochemistry/ Immunofluorescence: HSF1 Antibody (4B4) [NBP2-42206]

Immunocytochemistry/Immunofluorescence: HSF1 Antibody (4B4) [NBP2-42206] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 20x. Heat Shocked at 42C for 1h.

Applications for HSF1 Antibody (4B4) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:2000

Western Blot

1:1000
Application Notes
1 ug/ml of HSF1 Antibody was sufficient for detection of HSF1 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSF1

Human cells respond to heat stress by inducing the binding of a pre-existing transcriptional activator (heat shock factor, HSF) to DNA (1). Induction of heat shock protein (HSP) gene expression by stress is initiated by binding of HSF1 to HSP gene promoters to increase their transcription. The cytoprotective functions of these HSPs are essential for cell survival, and thus it is critical that inducible HSP gene expression be executed rapidly and efficiently. There is an interaction between heat shock factor 1 (HSF1) and symplekin, a protein known to form a complex with the polyadenylation factors CstF and CPSF. HSF1-symplekin complexes are detected only after stress treatment, and these two proteins co-localize in punctate nuclear structures in stressed cells (2). A chaperone/Hsp functioning as repressor of heat shock transcription factor (HSF) could make activation of hsp genes dependent on protein unfolding. It has been concluded that Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1 (3).

Long Name

Heat Shock Transcription Factor 1

Alternate Names

HSTF1

Gene Symbol

HSF1

UniProt

Q00613 (Human)

Additional HSF1 Products

Product Documents for HSF1 Antibody (4B4) - BSA Free

Certificate of Analysis

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Product Specific Notices for HSF1 Antibody (4B4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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