Human AMPK beta 1 Antibody

Detection of Human AMPK beta 1 by Western Blot. Western Blot shows lysates of K562 human chronic myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human AMPK beta 1 Monoclonal Antibody (Catalog # MAB11642) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for AMPK beta 1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of AMPK beta 1 in Human Kidney. AMPK beta 1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human AMPK beta 1 Monoclonal Antibody (Catalog # MAB11642) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human AMPK beta 1 by Simple Western^TM. Simple Western shows lysates of HEK293T human embryonic kidney cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for AMPK beta 1 at approximately 52 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human AMPK beta 1 Monoclonal Antibody (Catalog # MAB11642). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.