Human Axl Antibody (108724)

R&D Systems | Catalog # MAB154

R&D Systems
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, ELISA Capture (Matched Antibody Pair), Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Cell Selection, CyTof, ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 108724
View all Axl Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Axl
Met1-Pro440
Accession # AAA61243

Specificity

Detects human Axl in direct ELISAs. In direct ELISAs, this antibody shows no cross-reactivity with recombinant mouse Axl, recombinant human (rh) Dtk or rhMer.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Axl Antibody (108724)

Detection of Axl antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of Axl in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Axl Monoclonal Antibody (Catalog # MAB154, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Detection of Axl antibody in A431 Human Cell Line antibody by Flow Cytometry.

Detection of Axl in A431 Human Cell Line by Flow Cytometry.

A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Axl Monoclonal Antibody (Catalog # MAB154, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Axl Antibody Specificity is Shown by Flow Cytometry in Knockout Cell Line.

Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line.

Axl knockout A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Axl Monoclonal Antibody (Catalog # MAB154, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). No staining in the Axl knockout A431 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
Detection of Human Human Axl Antibody by Flow Cytometry

Detection of Human Human Axl Antibody by Flow Cytometry

MR766 infection of unstimulated and decidualized T-HESC cell line.(a) T-HESC (left) and decidualized (d) T-HESC (right) were stained with ZIKV anti-dsRNA or E protein mAbs whereas the nuclei were stained with Hoechst. (b) Surface expression of AXL (red) and MER (blue) in T-HESC (left) and dT-HESC (right) was determined in uninfected cells by flow cytometry. The histograms of one experiment representative of 3 independently performed are shown. Double immunostaining for ZIKV E protein and calreticulin (c) or dsRNA and vimentin (d) in dT-HESC either uninfected or infected with MR766 at 72 h post-infection; Hoechst was used to stain nuclei. Arrows indicate localization of E protein in areas occupied by calreticulin. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28281680), licensed under a CC-BY license. Not internally tested by R&D Systems.
Human Axl ELISA Standard Curve

Human Axl ELISA Standard Curve

Recombinant Human Axl Fc Chimera (Catalog # 154-AL) was serially diluted and captured by Mouse Anti-Human Axl Monoclonal Antibody (Catalog # MAB154) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Axl Antibody (108724)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line, or A431 human epithelial carcinoma cell line

Knockout Validated

Axl is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable in Axl knockout A431 cell line.

Human Axl Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human Axl Biotinylated Antibody (Catalog # BAF154)
  • Standard: Recombinant Human Axl Fc Chimera Protein, CF (Catalog # 154-AL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB154 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

References

  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.

Long Name

Axl Receptor Tyrosine Kinase

Alternate Names

AI323647, ARK, JTK11, Tyro7, UFO

Entrez Gene IDs

558 (Human); 26362 (Mouse); 102126361 (Cynomolgus Monkey)

Gene Symbol

AXL

UniProt

AAA61243

Additional Axl Products

Product Documents for Human Axl Antibody (108724)

Certificate of Analysis

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Product Specific Notices for Human Axl Antibody (108724)

For research use only

Citations for Human Axl Antibody (108724)

Customer Reviews for Human Axl Antibody (108724) (2)

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  • Human Axl Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 07/31/2020
    Human Axl Antibody (108724) MAB154
  • Human Axl Antibody
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: NCI-H1299
    Species: Human
    Verified Customer | Posted 06/07/2016
    Human Axl Antibody (108724) MAB154

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