Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair), Flow Cytometry

Cited:

Flow Cytometry, ELISA Capture, ELISA Detection

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Axl Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Axl
Met1-Pro440
Accession # AAA61243

Specificity

Detects human Axl in ELISAs and Western blots. In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant mouse Axl, recombinant human (rh) Dtk, and rhMer is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Axl Biotinylated Antibody

Detection of Axl in A431 cells by Flow Cytometry

A431 (filled histogram) and Jurkat (open histogram) cells were stained with Goat Anti-Human Axl Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF154, filled histogram) followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Axl Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: A431 human epithelial carcinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human Axl Fc Chimera (Catalog # 154-AL)

Human Axl Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human Axl Antibody (Catalog # MAB154)
  • Capture Reagent: Human Axl Antibody (Catalog # MAB154R)
  • Standard: Recombinant Human Axl Fc Chimera Protein, CF (Catalog # 154-AL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF154 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1,2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

References

  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.

Long Name

Axl Receptor Tyrosine Kinase

Alternate Names

AI323647, ARK, JTK11, Tyro7, UFO

Entrez Gene IDs

558 (Human); 26362 (Mouse); 102126361 (Cynomolgus Monkey)

Gene Symbol

AXL

UniProt

AAA61243

Additional Axl Products

Product Documents for Human Axl Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Axl Biotinylated Antibody

For research use only

Citations for Human Axl Biotinylated Antibody

Customer Reviews for Human Axl Biotinylated Antibody (1)

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  • Human Axl Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 08/26/2020
    Human Axl Biotinylated Antibody BAF154

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