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Human beta-3 Adrenergic R/ADRB3 Antibody

Catalog #: MAB9419
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB9419-100
MAB9419-SP

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Detection of beta ‑3 Adrenergic R/ADRB3 in HEK293 Human Cell Line Transfected with Human beta ‑3 Adrenergic R/ADRB3 and eGFP by Flow Cytometry.
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Human beta-3 Adrenergic R/ADRB3 Antibody Summary

Species Reactivity
Human
Specificity
Stains human beta -3 Adrenergic R/ADRB3 transfectants but not irrelevent transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 493324
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived transfected with human beta -3 Adrenergic R/ADRB3
Met1-Ser408
Accession # P13945
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of beta -3 Adrenergic R/ADRB3 antibody in HEK293 Human Cell Line Transfected with Human beta -3 Adrenergic R/ADRB3 and eGFP antibody by Flow Cytometry. View Larger

Detection of beta ‑3 Adrenergic R/ADRB3 in HEK293 Human Cell Line Transfected with Human beta ‑3 Adrenergic R/ADRB3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human beta -3 Adrenergic R/ADRB3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human beta -3 Adrenergic R/ADRB3 Monoclonal Antibody (Catalog # MAB9419) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry beta -3 Adrenergic R/ADRB3 antibody in Human Adipocytes by Immunocytochemistry (ICC). View Larger

beta ‑3 Adrenergic R/ADRB3 in Human Adipocytes. beta -3 Adrenergic R/ADRB3 was detected in immersion fixed human adipocytes using Mouse Anti-Human beta -3 Adrenergic R/ADRB3 Monoclonal Antibody (Catalog # MAB9419) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-3 Adrenergic R/ADRB3

 ADRB3 belongs to the G protein-coupled receptor 1 family of beta adrenergic receptors. Beta-adrenergic receptors, including ADRB3, mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. ADRB3 is involved in the regulation of lipolysis and thermogenesis. A variant of ADRB3 seems to be associated with weight gain (obesity).

Long Name
Beta-3 Adrenergic Receptor
Entrez Gene IDs
155 (Human); 11556 (Mouse); 25645 (Rat)
Alternate Names
ADRB3; ADRB3R; adrenergic, beta-3-, receptor; B3AR; beta-3 Adrenergic R; beta-3 adrenergic receptor; beta3 AdrenergicR; beta-3 AdrenergicR; Beta-3 adrenoceptor; Beta-3 adrenoreceptor; BETA3AR; FLJ99960

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Citation for Human beta-3 Adrenergic R/ADRB3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. beta3 adrenergic receptor as potential therapeutic target in ADPKD
    Authors: G Schena, M Carmosino, S Chiurlia, L Onuchic, M Mastropasq, E Maiorano, FP Schena, MJ Caplan
    Physiological Reports, 2021-10-01;9(20):e15058.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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