Human BMI-1 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF3334G Datasheet / COA / SDS

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AF3334G-100UG

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All BMI-1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BMI-1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human BMI-1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BMI-1
Asp96-Gly326
Accession # P35226

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BMI-1

BMI-1 (B cell-specific Moloney-MLV Integration site 1) is a 45 kDa protooncogene that is a class II member of the Polycomb group of genes. It participates in the formation of large multimeric complex termed PRC1 that inhibits target gene transcription loss of BMI-1 function and precludes stem cells from self-replicating. The amino acid sequence of human BMI-1 is 99%, 97%, 99%, and 99% identical to that of bovine, mouse, feline, and canine BMI-1, respectively.

Long Name

B Lymphoma Mo-MLV Insertion Region 1

Entrez Gene IDs

648 (Human); 12151 (Mouse)

Alternate Names

B lymphoma Mo-MLV insertion region 1 homolog (mouse); B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1; BMI-1; FLVI2/BMI1; MGC12685; murine leukemia viral (bmi-1) oncogene homolog; PCGF4; polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; RING finger protein 51; RNF51; RNF51Polycomb group RING finger protein 4