Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 303104
View all Carbonic Anhydrase IX/CA9 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX/CA9
Pro59-Asp414
Accession # Q16790

Specificity

Detects human Carbonic Anhydrase IX/CA9 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Carbonic Anhydrase IX/CA9 Antibody

Carbonic Anhydrase IX/CA9 antibody in Human Colon by Immunohistochemistry (IHC-P).

Carbonic Anhydrase IX/CA9 in Human Colon.

Carbonic Anhydrase IX/CA9 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human Carbonic Anhydrase IX/CA9 Monoclonal Antibody (Catalog # MAB21881) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Carbonic Anhydrase IX/CA9 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Reviewed Applications

Read 2 reviews rated 4.5 using MAB21881 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carbonic Anhydrase IX/CA9

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1‑3). CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)‑associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). The immunogen corresponds to the extracellular portion of human CA9.

References

  1. Pastorek, J. et al. (1994) Oncogene 9: 2877.
  2. Opavsky, R. et al. (1996) Genomics 33: 480.
  3. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  4. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
  5. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.

Alternate Names

CA9, G250, MN, RCC

Entrez Gene IDs

768 (Human); 230099 (Mouse)

Gene Symbol

CA9

UniProt

Q16790

Additional Carbonic Anhydrase IX/CA9 Products

Product Documents for Human Carbonic Anhydrase IX/CA9 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Carbonic Anhydrase IX/CA9 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human Carbonic Anhydrase IX/CA9 Antibody

Customer Reviews for Human Carbonic Anhydrase IX/CA9 Antibody (2)

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  • Human Carbonic Anhydrase IX/CA9 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Esophageal adenocarcinoma
    Species: Human
    Verified Customer | Posted 11/26/2021
    Human Carbonic Anhydrase IX/CA9 Antibody MAB21881
  • Human Carbonic Anhydrase IX/CA9 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: U87MG and CAOV4
    Species: Human
    Verified Customer | Posted 07/31/2018

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Protocols

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