Human CCL17/TARC Antibody

R&D Systems | Catalog # AF364

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CCL17/TARC Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL17/TARC (R&D Systems, Catalog # 364-DN)
Ala24-Ser94
Accession # Q92583

Specificity

Detects human CCL17/TARC in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse TARC is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL17/TARC Antibody

Chemotaxis Induced by CCL17/TARC and Neutralization by Human CCL17/TARC Antibody.

Chemotaxis Induced by CCL17/TARC and Neutralization by Human CCL17/TARC Antibody.

Recombinant Human CCL17/TARC (Catalog # 364-DN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL17/ TARC (0.01 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF364). The ND50 is typically 0.3-1.5 µg/mL.
CCL17/TARC antibody in Human Spleen by Immunohistochemistry (IHC-P).

CCL17/TARC in Human Spleen.

CCL17/TARC was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF364) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of CCL17/TARC by Western Blot

Detection of CCL17/TARC by Western Blot

Expression of CCL17 and the morphological transformation of renal tissues in UUO mice. (A) Kidney tissue of C57BL/6 mice with unilateral ureteral obstruction (UUO) for 7 and 14 days visualized by hematoxylin and eosin (H&E) stain, immunohistochemical stain (CCL-17, alpha -SMA, and vimentin), and Masson’s Trichrome staining; (B) qRTPCR and (C) western blot of mRNA and protein expressions of CCL17, collagen I, alpha -SMA, and vimentin in sham surgery and UUO mice. GAPDH was used to normalize mRNA loading. Results are expressed as the mean ± SD of at least three independent experiments. ** p < 0.01 compared with sham surgery group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34943853), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CCL17/TARC by Immunohistochemistry

Detection of CCL17/TARC by Immunohistochemistry

Expression of CCL17 and the morphological transformation of renal tissues in UUO mice. (A) Kidney tissue of C57BL/6 mice with unilateral ureteral obstruction (UUO) for 7 and 14 days visualized by hematoxylin and eosin (H&E) stain, immunohistochemical stain (CCL-17, alpha -SMA, and vimentin), and Masson’s Trichrome staining; (B) qRTPCR and (C) western blot of mRNA and protein expressions of CCL17, collagen I, alpha -SMA, and vimentin in sham surgery and UUO mice. GAPDH was used to normalize mRNA loading. Results are expressed as the mean ± SD of at least three independent experiments. ** p < 0.01 compared with sham surgery group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34943853), licensed under a CC-BY license. Not internally tested by R&D Systems.
Human CCL17 / TARC ELISA Standard Curve

Human CCL17 / TARC ELISA Standard Curve

Recombinant Human CCL17/TARC (Catalog # 364-DN) was serially diluted and captured by Mouse Anti-Human CCL17/TARC Monoclonal Antibody (Catalog # MAB364) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF364) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CCL17/TARC Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen

Western Blot

0.1 µg/mL
Sample: Recombinant Human CCL17/TARC (Catalog # 364-DN)

Neutralization

Measured by its ability to neutralize CCL17/TARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 0.01 µg/mL Recombinant Human CCL17/TARC.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL17/TARC

CCL17 is a novel CC chemokine identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid (aa)residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3, and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.

References

  1. Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
  2. Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
  3. Nomiyama, H. et al. (1997) Genomics 40:211.

Alternate Names

ABCD-2, SCYA17, TARC

Entrez Gene IDs

6361 (Human); 20295 (Mouse)

Gene Symbol

CCL17

UniProt

Q92583

Additional CCL17/TARC Products

Product Documents for Human CCL17/TARC Antibody

Certificate of Analysis

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Product Specific Notices for Human CCL17/TARC Antibody

For research use only

Citations for Human CCL17/TARC Antibody

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Protocols

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