Human CCL18/PARC Antibody

R&D Systems | Catalog # AF394

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CCL18/PARC Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL18/PARC
Ala21-Ala89
Accession # P55774

Specificity

Detects human CCL18/PARC in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) MIP-1 alpha and recombinant mouse MIP-1 alpha is observed and less than 10% cross-reactivity with rhMPIF‑1, recombinant viral (rv) MIP‑I, rvMIP‑II, rhHCC-1, and rhMIP-1 delta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CCL18/PARC Antibody

Human CCL18 / PARC ELISA Standard Curve

Human CCL18 / PARC ELISA Standard Curve

Recombinant Human CCL18/PARC (Catalog # 394-PA) was serially diluted and captured by Mouse Anti-Human CCL18/PARC Monoclonal Antibody (Catalog # MAB394) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL18/PARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF394) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of CCL18/PARC by Flow Cytometry

Detection of CCL18/PARC by Flow Cytometry

CCL18 produced by TAMs mediates the chemoresistance-inducing phenotype polarization in NBFs.A Representative images(left) and diameters(right) of collagen gel contraction assay for NBFs with or without CCL18 and TGF-beta treatment was determined by three-dimensional collagen matrices (n = 3). B, C QRT–PCR(B) and western blotting(C) for alpha -SMA, FAP, CD10 and GPR77 expression in NBFs with or without CCL18 and TGF-beta treatment (n = 3). D Representative flow cytometric analysis for CD10 and GPR77 in NBFs with indicated treatment (n = 3). E Representative western blotting for alpha -SMA, FAP, CD10, and GPR77 in NBFs cultured alone (UT) or co-cultured with PBMC or TAMs isolated from different subtypes of breast cancer (n = 3). F Western blotting for alpha -SMA, FAP, CD10 and GPR77 in NBFs cultured alone (UT) or co-cultured with PBMC or TAMs pretreated with control IgG or neutralizing antibodies against CCL18( alpha CCL18) or TGF-beta ( alpha TGF-beta ) (n = 3). G Representative images(left) and diameters(right) of collagen gel contraction assay for NBFs treated as in F (n = 3). Data expressed as mean ± SEM, **P < 0.01, ***P < 0.001 compared with untreated NBFs (A, B) or NBFs co-cultured with untreated TAMs (G) by two-tailed one-way ANOVA with Dunnett’s multiple comparison test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36418470), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CCL18/PARC Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Human monocyte-derived dendritic cells treated with Recombinant Human IL‑4 (Catalog # 204-IL), Recombinant Human GM-CSF (Catalog # 215-GM), Recombinant Human TNF‑ alpha (Catalog # 210-TA), Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB), and LPS, fixed with paraformaldehyde and permeabilized with saponin

Western Blot

0.1 µg/mL
Sample: Recombinant Human CCL18/PARC (Catalog # 394-PA)

Reviewed Applications

Read 3 reviews rated 4.7 using AF394 in the following applications:

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL18/PARC

CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10, and IL-13, and inhibited by IFN‑ gamma. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells but not monocytes or neutrophils.

References

  1. Adema, G. et al. (1997) Nature 387:713.
  2. Kodelja, V. et al. (1998) J. Immunol. 160:141.

Alternate Names

AMAC-1, DC-CK1, MIP-4, PARC

Entrez Gene IDs

6362 (Human)

Gene Symbol

CCL18

UniProt

P55774

Additional CCL18/PARC Products

Product Documents for Human CCL18/PARC Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL18/PARC Antibody

For research use only

Citations for Human CCL18/PARC Antibody

Customer Reviews for Human CCL18/PARC Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
33%
3 Stars
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1 Stars
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Showing  1 - 3 的 3 reviews Showing All
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  • Human CCL18/PARC Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 06/09/2020
    We used this antibody in an in-house ELISA along with mAb (394) and protein (394-PA-010) to detect CCL18 in human serum and plasma. This combination detected CCL18 in human serum and plasma efficiently and generated a good standard curve.
    Human CCL18/PARC Antibody AF394
  • Human CCL18/PARC Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2017
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23874339
    Species: Other
    Verified Customer | Posted 01/08/2015

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