Human CCL18/PARC Antibody [Biotin]

R&D Systems | Catalog # BAF394

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair), Intracellular Staining by Flow Cytometry

Cited:

Immunocytochemistry, Complex Application, ELISA Detection, ELISA Development (Detection), Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CCL18/PARC Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL18/PARC
Ala21-Ala89
Accession # P55774

Specificity

Detects human CCL18/PARC in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) HCC‑1, rhMPIF‑1, rhMIP‑1 alpha, recombinant mouse (rm) MIP‑1 alpha, rhMIP‑1 beta, rmMIP‑1 beta, recombinant viral (rv) MIP‑I, rvMIP‑II, and rhMIP‑1 delta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CCL18/PARC Antibody [Biotin]

Detection of CCL18/PARC antibody in Human Macrophages antibody by Flow Cytometry.

Detection of CCL18/PARC in Human Macrophages by Flow Cytometry.

Macrophages were generated from positively selected (MAGH105) CD14+ human PBMC, cultured with 50 ng/ml rhM-CSF (216-MC), 10 ng/ml rhIL-10 (217-IL) and 10 ng/ml rhIL-4 (204-IL) for 6 days, then treated with 3nM Monensin overnight and stained with (A) Goat Anti-Human CCL18/PARC Biotinylated Polyclonal Antibody (Catalog # BAF394) or (B) control antibody (BAF108), followed by Allophycocyanin-conjugated Streptavidin (F0050) and Mouse anti-Human CD163 Alexa Fluor® 488-conjugated Monoclonal Antibody (FAB1607G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
Human CCL18 / PARC ELISA Standard Curve

Human CCL18 / PARC ELISA Standard Curve

Recombinant Human CCL18/PARC (Catalog # 394-PA) was serially diluted and captured by Mouse Anti-Human CCL18/PARC Monoclonal Antibody (Catalog # MAB394) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL18/PARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF394) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CCL18/PARC Antibody [Biotin]

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Human macrophages generated from positively selected (Catalog # MAGH105) CD14+ PBMC cultured with Recombinant Human M-CSF (Catalog # 216-MC), Recombinant Human IL-10 (Catalog # 217-IL) and  Recombinant Human IL‑4 (Catalog # 204-IL), fixed with paraformaldehyde and permeabilized with saponin

Western Blot

0.1 µg/mL
Sample: Recombinant Human CCL18/PARC (Catalog # 394-PA)

Human CCL18/PARC Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human CCL18/PARC Antibody (Catalog # MAB394)
  • Standard: Recombinant Human CCL18/PARC Protein (Catalog # 394-PA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.3 using BAF394 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL18/PARC

CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10, and IL-13, and inhibited by IFN‑ gamma. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells but not monocytes or neutrophils.

References

  1. Adema, G. et al. (1997) Nature 387:713.
  2. Kodelja, V. et al. (1998) J. Immunol. 160:141.

Alternate Names

AMAC-1, DC-CK1, MIP-4, PARC

Entrez Gene IDs

6362 (Human)

Gene Symbol

CCL18

UniProt

P55774

Additional CCL18/PARC Products

Product Documents for Human CCL18/PARC Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL18/PARC Antibody [Biotin]

For research use only

Citations for Human CCL18/PARC Antibody [Biotin]

Customer Reviews for Human CCL18/PARC Antibody [Biotin] (4)

4.3 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
75%
3 Stars
0%
2 Stars
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1 Stars
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  • Name: Anonymous
    Application: ELISA
    Sample Tested: See PMID: 19120345
    Species: Human
    Verified Customer | Posted 01/14/2015
  • Name: Anonymous
    Application: ELISA
    Sample Tested: See PMID: 21801246
    Species: Human
    Verified Customer | Posted 01/14/2015
  • Name: Anonymous
    Application: ELISA
    Sample Tested: See PMID: 19175890
    Species: Human
    Verified Customer | Posted 01/14/2015
  • Name: Anonymous
    Application: ELISA
    Sample Tested: See PMID 22280057
    Species: Human
    Verified Customer | Posted 01/13/2015

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