Human CD117/c-kit Antibody
Human CD117/c-kit Antibody Summary
Accession # P10721
Human CD117/c-kit Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by SCF/c‑kit Ligand and Neutralization by Human CD117/c-kit Antibody. Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/c-kit Ligand (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD117/c-kit Monoclonal Antibody (Catalog # MAB332). The ND50 is typically 0.5-2.5 µg/mL.
Detection of Human CD117/c-kit by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line. PVDF membrane was probed with 1.0 µg/mL of Mouse Anti-Human CD117/c-kit Monoclonal Antibody (Catalog # MAB332) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD117/c-kit at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human CD117/c-kit by Simple WesternTM. Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 172 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human CD117/c-kit Monoclonal Antibody (Catalog # MAB332). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Stem cell factor receptor (CD117, the gene product of the c-kit proto-oncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis, and hematopoiesis. The human stem cell factor receptor cDNA encodes a 972 amino acid (aa) residue precursor membrane protein with a 25 aa residue signal peptide (experimentally determined), a 495 aa residue extracellular domain, a 23 aa residue transmembrane segment, and a 429 aa residue cytoplasmic domain. Stem cell factor receptor is a member of the type III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, Flt-3, PDGF, and VEGF. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. SCF binding induces receptor homodimerization and signal transduction. SCF receptor is expressed in hematopoietic progenitor cells, normal B and T lymphocyte progenitor cells, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung, and gut. In addition, SCF receptor expression has also been reported in a number of human tumor cell lines. SCF receptor can be proteolytically cleaved from the cell surface and high levels of soluble SCF receptor has been detected in cell conditioned medium and human plasma. Recombinant soluble SCF receptor binds SCF with high affinity and is a potent SCF antagonist.
- Broudy, V. (1997) Blood 90:1345.
- Vliagoftis, H. et al. (1997) J. Allergy Clin. Immunol. 100:435.
Citations for Human CD117/c-kit Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Desensitization of human breast progenitors by a transient exposure to pregnancy levels of estrogen
Authors: L Rønnov-Jes, J Kim, N Goldhammer, MC Klitgaard, M Smicius, MB Bechmann, R Villadsen, OW Petersen
Scientific Reports, 2021-08-26;11(1):17232.
Sample Types: Cell Lysates
Applications: Western Blot
EXEL-0862, a novel tyrosine kinase inhibitor, induces apoptosis in vitro and ex vivo in human mast cells expressing the KIT D816V mutation.
Authors: Pan J, Quintas-Cardama A, Kantarjian HM, Akin C, Manshouri T, Lamb P, Cortes JE, Tefferi A, Giles FJ, Verstovsek S
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot
Modulation of GM-CSF release by enantiomers of beta-agonists in human airway smooth muscle.
Authors: Ameredes BT, Calhoun WJ
J. Allergy Clin. Immunol., 2005-07-01;116(1):65-72.
Sample Types: Whole Cells
Applications: Flow Cytometry
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